Addgene

We narrowed to 1,777 results for: tet on vector

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pTER SADB shRNA Human

Plasmid

#67151

Purpose

Doxycycline-regulated mammalian expression vector for expressing shRNA against human SADB

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

BRSK1 (BRSK1 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1 Tet

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS_KDR_111del

Plasmid

#65659

Purpose

FLAG-HA expression vector with RBPMS carrying deletion at amino acid 111 (1-111) and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

introduced stop codon such as to produce deletion…

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-dCas9

Plasmid

#218371

Purpose

Expression of dCas9 for CRISPR-interference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

dCas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PtetA

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGPS21loxFRTNeo

Plasmid

#27180

Depositor

Yoh Wada

Article

Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Insert

lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R

Use

In vitro transposion

Tags

Expression

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pKI1.1R

Plasmid

#85808

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Expression

Plant

Mutation

Promoter

AtRPS5A

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pKIR1.1

Plasmid

#85758

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG

Expression

Plant

Mutation

Promoter

AtRPS5A

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::tTA2-bGHpA

Plasmid

#62448

Purpose

MXS_chaining vector with PGK::tTA2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::rtTA3-bGHpA

Plasmid

#62445

Purpose

MXS_chaining vector with CMV::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with CMV promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::rtTA3-bGHpA

Plasmid

#62446

Purpose

MXS_chaining vector with PGK::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::tTA2-bGHpA

Plasmid

#62447

Purpose

MXS_chaining vector with CMV::tTA2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tetracycline transactivator with CMV promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pRPF185

Plasmid

#106367

Purpose

C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

Depositor

Robert Fagan, Neil Fairweather

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Term(fdx)-Ptet-gusA-Term(slpA) (gusA )

Use

E. coli - c. difficile shuttle vector

Tags

Expression

Mutation

codon optimised for C. difficile

Promoter

Ptet

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pTS2163

Plasmid

#169632

Purpose

Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

OTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3

Use

Tags

Expression

Mammalian

Mutation

Promoter

tetO7-PhCMVmin / PPGK

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRAN334

Plasmid

#120814

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

Depositor

David S. Weiss, Craig Ellermeier

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBluescript-Q22YU3-KO

Plasmid

#170313

Purpose

Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22YU3 with Paromomycin selection

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Q22YU3 knockout

Use

Tetrahymena somatic gene knockout vector

Tags

Expression

Mutation

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescript-Q22MS1-KO

Plasmid

#170312

Purpose

Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selection

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Q22MS1 knockout

Use

Tetrahymena somatic gene knockout vector

Tags

Expression

Mutation

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pB-TR-hCAII

Plasmid

#232480

Purpose

Tetracycline inducible PiggyBac vector expressing human CAII gene including flag-tag (DYKDDDDK) on the 3' end

Depositor

Lukas Trantirek

Article

Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

Insert

Human carbonic anhydrase II (CA2 Human)

Use

Transposon system

Tags

DYKDDDDK (FLAG-tag)

Expression

Mammalian

Mutation

Promoter

CMV-TetO

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSUP202pol4

Plasmid

#67184

Purpose

Conjugative cloning vector

Depositor

Julia Vorholt, Hauke Hennecke

Article

Fischer et al EMBO J. 1993 Jul;12(7):2901-12.

Type

Empty backbone

Use

Conjugative bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pMP4657

Plasmid

#107773

Purpose

Expression of EGFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EGFP in pME6010

Use

Tags

pLacZ-EGFP

Expression

Bacterial

Mutation

Promoter

pLacZ

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMP4639

Plasmid

#107771

Purpose

Expression of EYFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EYFP in pME6010

Use

Tags

pLacZ-EYFP

Expression

Bacterial

Mutation

Promoter

pLacZ

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKF-P14MM2AG5h

Plasmid

#128254

Purpose

Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

rtTA-Advanced::P2A::EGFP

Use

Synthetic Biology; Flp-in expression vector

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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