We narrowed to 16,261 results for: LEA
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Plasmid#75236PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hH4-AVITEV
Plasmid#74051Purposeretroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman Histone-H4 (H4C16 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianPromoterpMSCV-LTRsAvailable SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO5-SgHottip-GFP-CRISPRa
Plasmid#134990PurposedCas9-mediated activation of HOTTIP in mammalian cellsDepositorAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1-V1251R_R
Plasmid#147376PurposeMammalian Expression of HsNot1iso1-V1251RDepositorInsertHsNot1iso1-V1251R (CNOT1 Human)
ExpressionMammalianMutationA deletion of 5 AA 822-827 SKMKPS-> T and one …Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-FUSN-miRFP670-Cry2WT
Plasmid#122441PurposeExpresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertFUS (FUS Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationContains only amino acids 1-214PromoterSFFVAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_CRF1.0
Plasmid#208662PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
UseAAVPromoterEFSAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)
Plasmid#208972PurposeRecruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertePhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75237PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA
Plasmid#210503Purposeexpresses LAUNCHERsbmv component in mammalian cellsDepositorInsertPDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA
TagsMycExpressionMammalianPromoterCMVAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 myc-mβTrCP WD1-7 (241-569)
Plasmid#62977PurposeExpresses myc-tagged mouse βTrCP residues 241-569DepositorAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE
Plasmid#174134PurposepAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoterDepositorInsertNos1ap (Nos1ap Mouse)
UseAAVTags3xFLAG and mCherryMutationOnly contains the sequences coding for amino acid…PromoterhSynAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
mature ISG15 C-term. domain (79-157)
Plasmid#110764PurposeBacterial expression of proISG15 C-term. domainDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE GFP-Ago2 S387E
Plasmid#60655PurposeGFP-Ago2 S387E mutant lentiviral vectorDepositorAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-CtIP-siR-S664A-S679A-S745A
Plasmid#82031PurposePlasmid for constitutive or doxy-inducible expression of S664A+S679A+S745A human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertCtIP (RBBP8 Human)
TagsHAExpressionMammalianMutationNon phosphorylatable mutations on 3 of the main A…PromoterCMV-tetAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
proISG15 C-term. domain (79-165)
Plasmid#110763PurposeBacterial expression of proISG15 C-term. domainDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR
Plasmid#196518PurposeExpression of Ctdnep1_D67E-GFPDepositorInsertCtdnep1_D67EsiR (CTDNEP1 Human)
TagseGFPExpressionMammalianMutationD67E mutation (phosphastase dead). Silent mutatio…PromoterCMV promoter; T7 promoterAvailable SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-HsSyx3
Plasmid#179288PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage siteDepositorInsertSyx
TagsHis6 + SUMO + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CACNA1F_L (OZ507)
Plasmid#26957DepositorInsertZinc finger array targeting cacna1f (cacna1f Zebrafish)
UseZebrafish targetingAvailable SinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCU-tDimer
Plasmid#181865PurposeExpresses tDimer-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoterDepositorInsertmitochondrial calcium uniporter (Mcu Mouse)
UseAAV and AdenoviralTagstDimerExpressionMammalianPromoterCaMKIIaAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-shCasp2-IRES-GFP
Plasmid#52061Purposestable knockdown of Caspase-2 in mammalian cellsDepositorAvailable SinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only