We narrowed to 17,340 results for: EMB

1. pGL3-basic-Polr2a promoter_600bps

   Plasmid

   #21111

   Depositor

   Dean Tantin

   Article

   Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

   Insert

   Polr2a promoter_600bps (POLR2A Human)
   

   Expression

   Mammalian

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pFP.R400

   Plasmid

   #138175

   Purpose

   Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc-sgRNA-Cas9.scr_2

   Plasmid

   #190704

   Purpose

   Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Scrambled sgRNA 2
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   Drosophila U6

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNS1

   Plasmid

   #131769

   Purpose

   PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

   Depositor

   Jared Toettcher

   Article

   Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

   Insert

   FUSN_Fusion Red_Cry2WT
   

   Use

   Integration into δ-sites

   Expression

   Yeast

   Promoter

   PADH1

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRF2-HA

   Plasmid

   #200209

   Purpose

   Expression of full length mTRF2

   Depositor

   Sheila Teves

   Article

   Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

   Insert

   TRF2-HA (Terf2 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   EF_1alpha

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOPO SV40

   Plasmid

   #68455

   Purpose

   TOPO construct expressing SV40 for generation of GMAP-compatible promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Simian Virus Early 40 Promoter
   

   Use

   Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS33: pTwinStrep-SUMO-PmcTnsD

   Plasmid

   #168166

   Purpose

   PmcTnsD expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   PmcCAST TnsD
   

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TAL2274

   Plasmid

   #36711

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   MDM2-TALEN_Left (Mdm2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pR540

   Plasmid

   #207885

   Purpose

   template for 2 gRNA amplification

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   scaffold-pU6
   

   Expression

   Bacterial

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS021

    Plasmid

    #207917

    Purpose

    Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKE013

    Plasmid

    #207916

    Purpose

    Level 0 for gRNA in E1 module (5' CTGC-3' CCAT)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. psiCHECK-2-Zfp36-3'UTR

    Plasmid

    #61780

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Zfp36

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Zfp36 3'UTR
    

    Use

    Luciferase

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pET15b+PCNA1 P186C

    Plasmid

    #66173

    Purpose

    Expresses the P186C mutant of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    P186C

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTPI1-EMC1-GFP-APEX2-URA3

    Plasmid

    #184776

    Purpose

    Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    APEX2
    

    Expression

    Yeast

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. C514-E47: attB5r-Halotag-nostop-attB1r

    Plasmid

    #162963

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Halotag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-INPP5J

    Plasmid

    #171949

    Purpose

    Expresses INPP5J in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    INPP5J (INPP5J Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMaCTag-P17

    Plasmid

    #120028

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mEos4b
    

    Use

    Pcr template

    Tags

    mEos4b

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p5xFbx15 (-544/-527)-TK-Luc

    Plasmid

    #15686

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    5 x Fbx15 enhancer (Oct/Sox binding site)
    

    Use

    Luciferase

    Mutation

    Wild-type

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

19. mRuby2-AKTIP

    Plasmid

    #171942

    Purpose

    Expresses AKTIP in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    AKTIP (AKTIP Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOTF3-AK (clone3)

    Plasmid

    #21165

    Depositor

    James Thomson

    Article

    Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

    Insert

    POUF5F1 (genomic DNA) (POU5F1 Human)
    

    Use

    Cre/Lox; Homologous recombination

    Mutation

    NeoR, EGFP, and SV40 polyA are between the two ge…

    Available Since

    June 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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