We narrowed to 17,340 results for: EMB

1. UG75-ADH1-DGK1-3HA

   Plasmid

   #198595

   Purpose

   DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   DGK1 (DGK1 Budding Yeast)
   

   Tags

   3HA

   Expression

   Yeast

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. UG75-ADH1-PAH1-3HA

   Plasmid

   #198597

   Purpose

   PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   PAH1 (PAH1 Budding Yeast)
   

   Tags

   3HA

   Expression

   Yeast

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMaCTag-P01

   Plasmid

   #120012

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   moxBFP
   

   Use

   Pcr template

   Tags

   moxBFP

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABF2-2EGFP-ter-URA3

   Plasmid

   #184768

   Purpose

   Marker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   ABF2 (ABF2 Budding Yeast)
   

   Tags

   2EGFP

   Expression

   Yeast

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. C413-E22: attB4-polyhedrinp>-attB1r

   Plasmid

   #162922

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. iRFP713-FRB-Mss4-Kina

   Plasmid

   #202744

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)
   

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYFP (GB0053)

   Plasmid

   #68197

   Purpose

   Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   YFP coding region
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBGSA FPR1

   Plasmid

   #62600

   Purpose

   Constitutive expression of FPR1 in mammalian cells

   Depositor

   Heini Miettinen

   Article

   Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

   Insert

   FPR1 (FPR1 Human)
   

   Expression

   Mammalian

   Promoter

   SRalpha

   Available Since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

9. phNFL-Q332P

   Plasmid

   #132599

   Purpose

   Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E Q332P mutation

   Depositor

   Anthony Brown

   Article

   Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

   Insert

   Nefl (Nefl Human)
   

   Expression

   Mammalian

   Mutation

   995A>C;Gln332Pro

   Promoter

   CMV

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3_omega2

    Plasmid

    #68239

    Purpose

    GoldenBraid level 1 Omega plant expression vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PIGAZFN-R2

    Plasmid

    #37800

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    PIGA zinc finger R2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFP.R375

    Plasmid

    #138245

    Purpose

    Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTol2-HybridBarcode

    Plasmid

    #174037

    Purpose

    For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

    Depositor

    James Gagnon

    Article

    Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

    Insert

    CRISPR target sites
    

    Use

    Tol2 transgenesis

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKI-cjVASA-tdTomato

    Plasmid

    #160872

    Purpose

    Marmoset Targeting

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

    Insert

    tdTomato
    

    Use

    Marmoset targeting

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMaCTag-P32

    Plasmid

    #120043

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    S-Tag
    

    Use

    Pcr template

    Tags

    S-Tag

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMaCTag-Z25

    Plasmid

    #120068

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    YFP-LID
    

    Use

    Pcr template

    Tags

    YFP-LID

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. C455-E01: attB4-stuffer-attB5

    Plasmid

    #162983

    Purpose

    Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. C515-E01: attB5r-stuffer-attB1r

    Plasmid

    #162968

    Purpose

    Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. P16ΔS:S10-(MCS)-3xHA

    Plasmid

    #108183

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    P16ΔS

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-TGF beta type I receptor (mL45)

    Plasmid

    #34701

    Depositor

    Ying Zhang

    Article

    Yu et al EMBO J. 2002 Jul 15;21(14):3749-59.

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N265A, D267A, N268A

    Promoter

    CMV

    Available Since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only