We narrowed to 18,098 results for: LEA

1. FUG-T2A-Pten

   Plasmid

   #140738

   Purpose

   Lentiviral vector to overexpress human PTEN and GFP

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-PTA-PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Promoter

   hUbiC

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-SUMO-hDDI2-D252N_pET11a

   Plasmid

   #169122

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hDDI (DDI2 Human)
   

   Expression

   Bacterial

   Mutation

   D252N

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-sCre

   Plasmid

   #55635

   Purpose

   Expresses sCre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-eSpCas9-plus-NLS-6xHis

   Plasmid

   #207380

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, R1060A, amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-BG505.SOSIP-TM

   Plasmid

   #111844

   Purpose

   Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 Env (BG505 SOSIP.664)
   

   Tags

   CD5 leader sequence and TM helix from MHC class I

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; has SOSIP mutatio…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. 2_T7-mScarlet3-KRas

   Plasmid

   #225929

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3-KRas
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNS38-SadCas9-SNAP

   Plasmid

   #113718

   Purpose

   Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

   Depositor

   Gerald Schwank

   Article

   Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA-2xNLS-SNAP-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   D10A, N580A

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTU422

   Plasmid

   #178982

   Purpose

   13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Inserts

   gRAMP
   CRISPR array
   

   Tags

   Strep-Tag II SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PD-L1-miSFIT-Ce67

   Plasmid

   #124686

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-L1 (CD274 Human)
   

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS2155

    Plasmid

    #141065

    Purpose

    AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

    Insert

    SYN1-EmGFP-WPRE
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    April 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCMV_UdgX-APOBEC1-UdgX-nCas9

    Plasmid

    #163555

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-DHFR F[3]-N (LEU2)

    Plasmid

    #177798

    Purpose

    Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_UdgX-APOBEC1-UdgX-HF-nCas9

    Plasmid

    #163559

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-HF-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PD-1-miSFIT-1x

    Plasmid

    #124679

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PEA1

    Plasmid

    #176527

    Purpose

    Delivers all prime editing nickase components in a single plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CbH for Cas9, hU6 for gRNAs

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOTTC414 - pAAV EF1a hPREP

    Plasmid

    #59967

    Purpose

    An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    Prolyl oligopeptidase (PREP Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP/golgin-160/3DE

    Plasmid

    #180631

    Purpose

    Expression of caspase-resistant golgin-160

    Depositor

    Carolyn Machamer

    Article

    Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

    Insert

    golgin-160/3DE (GOLGA3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D59E, D139E, D311E

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. ROSA26(MultiFPsΔPuro)

    Plasmid

    #140759

    Purpose

    Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).

    Depositor

    Hideyuki Okano

    Article

    Serizawa et al Development. 2019 Nov 4;146(21). pii: de

    Inserts

    puromycin-N-acetyltransferase
    Venus
    mCherry
    mCerulean
    

    Use

    Mouse Targeting

    Promoter

    CAGGS

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. px459 sgAtg5

    Plasmid

    #175023

    Purpose

    CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

    Depositor

    David Tumbarello

    Article

    Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

    Insert

    ATG5 (ATG5 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSynapsin_NLS_GFP_P2A_SNAP_NR1

    Plasmid

    #170458

    Purpose

    Expresses SNAP tagged NR1 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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