We narrowed to 13,392 results for: mpl

1. Flag Depdc5 pRK5

   Plasmid

   #46340

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Differs from Depdc5 isoform 1 by 10 amino acids …

   Promoter

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Solo-mStayGold-CETN2

   Plasmid

   #227292

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CETN2 Homology Arms flanking a mStayGold Tag (CETN2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRN3P-HA-Suv4-20h1mut

   Plasmid

   #86690

   Purpose

   For transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

   Insert

   histone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)
   

   Use

   Tags

   HA

   Expression

   Mutation

   mutated region NHDC (asparagine 273 to cysteine 2…

   Promoter

   T3

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-EGFP-AID-BubR1

   Plasmid

   #47330

   Purpose

   for plasmid integration using the Flp-In system. AID at N-terminal

   Depositor

   Don Cleveland

   Article

   Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

   Insert

   BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
   

   Use

   Tags

   AID and GFP

   Expression

   Mammalian

   Mutation

   siRNA resistant

   Promoter

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

   Plasmid

   #170318

   Purpose

   Expresses FLAG and EGFP tagged RPL22, puromycin resistance

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD649-HAsp-SEMA3F-COMP5AP-AviTag-9xHis

   Plasmid

   #157541

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA3F (SEMA3F Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3-EMTB-TurboID-V5

   Plasmid

   #190736

   Purpose

   Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   ensconsin (MAP7 Human)
   

   Use

   Tags

   Myc, TurboID, and V5

   Expression

   Mammalian

   Mutation

   Microtubule binding-domain of ensconsin (amino ac…

   Promoter

   CMV

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-N-Solo-mStayGold-CENPA

   Plasmid

   #227283

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CENPA Homology Arms flanking a mStayGold Tag (CENPA Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-Solo-mStayGold-CANX

   Plasmid

   #227280

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CANX Homology Arms flanking a mStayGold Tag (CANX Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TetO-FUW-BICRAL-FLAG-pgk-puro

    Plasmid

    #107732

    Purpose

    Tet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORF

    Depositor

    Emily Dykhuizen

    Article

    Alpsoy et al J Biol Chem. 2018 Jan 26. pii: RA117.001

    Insert

    BICRAL (BICRAL Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo

    Plasmid

    #170319

    Purpose

    Expresses HA and EGFP tagged RPL22, neomycin resistance

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5FRT/Flag-FLJ10081

    Plasmid

    #64775

    Purpose

    Mammalian expression of Flag-tagged FLJ10081 (NSL3)

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    FLJ10081 (KANSL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)

    Plasmid

    #159579

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. RP1B FUS 1-163

    Plasmid

    #127192

    Purpose

    Protein expression for affinity purification

    Depositor

    Nicolas Fawzi

    Article

    Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

    Insert

    FUS 1-163 (FUS Human)
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-AuroraB kd K106R

    Plasmid

    #108493

    Purpose

    expression of EGFP-AuroraB kinase dead (K106R)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Kinase death K106A

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MBP-hnRNPA2_FL_WT

    Plasmid

    #98662

    Purpose

    Expresses MBP-tagged full length hnRNPA2

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET41b+_Ufd1-His

    Plasmid

    #117107

    Purpose

    Production of recombinant Ufd1-His in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Ufd1 (UFD1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-MBP human SPOP (aa 28-337)

    Plasmid

    #52294

    Purpose

    bacterial expression of human SPOP (aa 28-337) fused to His-MBP

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Insert

    SPOP (SPOP Human)
    

    Use

    Tags

    His-MBP and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pENTR221-YAP1 CA

    Plasmid

    #79502

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    3XFLAG

    Expression

    Mutation

    S127A

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Epac-S-H81

    Plasmid

    #170339

    Purpose

    GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

    Insert

    GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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