We narrowed to 14,951 results for: pET

1. mApple-ERM

   Plasmid

   #217377

   Purpose

   ER marker

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   rat cb5 ERM
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCLX-M38s/TOP-dGFP

   Plasmid

   #114295

   Purpose

   Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   dGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   destabilized GFP

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SNAP-23

   Plasmid

   #186686

   Purpose

   Expresses SNAP-23 in E. Coli

   Depositor

   Axel Brunger

   Article

   Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

   Insert

   SNAP23
   

   Use

   Tags

   GST and thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCaUra3-CUbo-AID*-GFP

   Plasmid

   #212775

   Purpose

   C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT3TS-Cre

   Plasmid

   #47117

   Purpose

   Can be used to in vitro transcribe Cre recombinase.

   Depositor

   Stephen Ekker

   Article

   Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

   Insert

   Cre
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Lac

   Available since

   Aug. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pconst #5-RFP

   Plasmid

   #81079

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Paul Adams, Jay Keasling

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23110

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPDS1-mEGFP-IRES-puro2b

   Plasmid

   #26464

   Depositor

   Daniel Gerlich

   Article

   Held et al Nat Methods. 2010 Sep . 7(9):747-54.

   Insert

   pPDS1 (PTTG1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKXUa1-HA-ATBF1

   Plasmid

   #40926

   Depositor

   Jin-Tang Dong

   Article

   Dong et al J Biol Chem. 2010 Oct 22;285(43):32801-9

   Insert

   ATBF1 (ZFHX3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pAc5.1B-EGFP-DmGIGYF_AH

   Plasmid

   #148918

   Purpose

   Insect Expression of DmGIGYF

   Depositor

   Elisa Izaurralde

   Article

   Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

   Insert

   DmGIGYF (Gyf Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SENP2M497A

    Plasmid

    #61088

    Purpose

    cleave the SUMO off when use PETSUMO vector for express fusion protein

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    SENP2M497A catalytic domain (SENP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M497A; Catalytic domain is residues 364-589

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pYFAC

    Plasmid

    #168982

    Purpose

    Filamentous fungi vector. Also referred to as pYFAC-pyrG.

    Depositor

    Yit Heng Chooi

    Article

    Chooi et al Environ Microbiol. 2017 May;19(5):1975-1

    Type

    Empty backbone

    Use

    Synthetic Biology; Heterologous expression in fil…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSB700 HL1gR4

    Plasmid

    #124450

    Purpose

    Expresses gRNA HL1 gR4 in the pSB700 backbone

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    HL1 gR4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pconst #4-RFP

    Plasmid

    #81078

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23107

    Available since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEM-sgMUC4_dual-MTS-3x-Set I

    Plasmid

    #162762

    Purpose

    Separately expressing 3 unique sgMUC4_dual-MTS (SetI)

    Depositor

    Antony K Chen

    Article

    Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

    Insert

    sgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAFNF-tdTomato

    Plasmid

    #125575

    Purpose

    Flp-dependent expression of tdTomato in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pconst #1-RFP

    Plasmid

    #81075

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Paul Adams, Jay Keasling

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23119

    Available since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRF2-HA

    Plasmid

    #200209

    Purpose

    Expression of full length mTRF2

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TRF2-HA (Terf2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCA528-AMOT(1-300)-sortase

    Plasmid

    #166706

    Purpose

    expresses AMOT(1-300) with C-terminal sortase tag in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    AMOT aa 1-300
    

    Use

    Tags

    His-SUMO and Sortase tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pR540

    Plasmid

    #207885

    Purpose

    template for 2 gRNA amplification

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    scaffold-pU6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS021

    Plasmid

    #207917

    Purpose

    Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge