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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,924 results for: LEA

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Showing: 7761 - 7780 of 13924 results

  1. TBXTA-c020

    Plasmid
    #139757
    Purpose
    For protein expression/crystallography (PDB 5QS0)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-N211
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139757-plasmid-map-sequence-id-296518

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  2. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid
    #125199
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125199-plasmid-map-sequence-id-243587

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  6. 6CKO (DOT1L)

    Plasmid
    #119962
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 1, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119962-plasmid-map-sequence-id-234358

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  10. Myc-gamma3 (in pCDNA3.1)

    Plasmid
    #118041
    Purpose
    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG3 (Gabrg3 Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    118041-plasmid-map-sequence-id-228642

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  11. Myc-gamma2s-EGFP

    Plasmid
    #118040
    Purpose
    GABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG2 (Gabrg2 Rat)
    Tags
    EGFP and cMyc epitope EQKLISEEDL inserted between…
    Expression
    Mammalian
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    118040-plasmid-map-sequence-id-228639

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  12. Myc-gamma1 (in pCDNA3.1)

    Plasmid
    #118038
    Purpose
    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG1 (Gabrg1 Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118038-plasmid-map-sequence-id-228560

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  13. Myc-gamma3 (in pCAGGS)

    Plasmid
    #118042
    Purpose
    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG3 (Gabrg3 Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118042-plasmid-map-sequence-id-228557

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  15. phSOD1-E100G-N’His

    Plasmid
    #117706
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    E100G
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117706-plasmid-map-sequence-id-227528

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  16. phSOD1-I113T-N’His

    Plasmid
    #117703
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    SOD1 (SOD1 Human)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    I113T
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    117703-plasmid-map-sequence-id-227513

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  17. NUDT4 (NUDT4A-c001)

    Plasmid
    #98241
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT4A (NUDT4 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98241-plasmid-map-sequence-id-193419

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  18. p5A3-T

    Plasmid
    #92019
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Publication
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with N-terminal TEV cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92019-plasmid-map-sequence-id-190012

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  19. p5A7-T

    Plasmid
    #92020
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Publication
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Mutation
    S219V
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92020-plasmid-map-sequence-id-190036

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Showing: 7761 - 7780 of 13924 results