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Plasmid#49942PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertRHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shDyn1
Plasmid#132711PurposeEncodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Pdyn geneDepositorInsertshDyn(1) (Pdyn Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shCrh1
Plasmid#132709PurposeEncodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Crh geneDepositorInsertshCrh(1) (Crh Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCVL.SSA TLR (Ani)
Plasmid#45577PurposeCodes for the SSA-TLR with the target site for I-Ani I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Ani I TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded I-Ani I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo G-Linker nsp1
Plasmid#175426PurposeExpresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertG-linker nsp1
UseTags3XFLAG HaloExpressionMammalianMutationG-linker in place of the RNase destabilization do…PromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo R119A K120A nsp1
Plasmid#175428PurposeExpresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertnsp1 R119A K120A
UseTags3XFLAG HaloExpressionMammalianMutationN-terminal mutants R119A K120APromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo E55A E57A K58A nsp1
Plasmid#175427PurposeExpresses E55A E57A K58A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertnsp1 E55A E57A K58A
UseTags3XFLAG HaloExpressionMammalianMutationN-terminal mutants E55A E57A K58APromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 3xFLAG Halo E36A E37A nsp1
Plasmid#175429PurposeExpresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertnsp1 E36A E37A
UseTags3XFLAG HaloExpressionMammalianMutationN-terminal mutants E36A E37APromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
ID1GFP Donor
Plasmid#119282PurposeMouse ID1-eGFP for HR to create reporterDepositorInsertId1GFP + homology arms (Id1 Mouse)
UseReporterTagseGFPExpressionMutationPromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shCrh2
Plasmid#132710PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh geneDepositorInsertshCrh(2) (Crh Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His
Plasmid#99743PurposeExpresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.DepositorInsertStx3 (STX3 Human)
UseTagsmyc-myc-hisExpressionMammalianMutationChanged both L165 and E166 to AlaninePromoterCMVAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSN17
Plasmid#100104Purposeexpresses a chimeric OCT4-repressor fusionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLOX-gfp-iresTK
Plasmid#12243DepositorInsertGFP, HSV1-TK
UseCre/Lox and LentiviralTagsExpressionMammalianMutationLoxP in 3' LTR.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
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EC10161
Plasmid#154060PurposeLevel 0.5 Golden Gate backbone, containing loxP sites.DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterN/AAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-RL-5BoxB
Plasmid#115364PurposeExpression vector to produce humanized Renilla luciferase with 5BoxB sequences in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
PM-GFP
Plasmid#21213DepositorInsertPM-GFP
UseTagsGFPExpressionMammalianMutationPM targetting sequence is Lyn (MGCIKSKRKD) (Lab …PromoterAvailable sinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only