We narrowed to 2,268 results for: plasmid for e coli

1. pJ02B2Rm_AE

   Plasmid

   #66059

   Purpose

   MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   FACS Controls
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPR220.08

   Plasmid

   #78553

   Purpose

   Plasmid constitutively expressing phycocyanobilin (PCB) biosynthesis genes and UirS sensor kinase gene

   Depositor

   Jeffrey Tabor

   Article

   Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

   Insert

   uirS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   J23108

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNO183

   Plasmid

   #101068

   Purpose

   ppsR2-bphP1 bicistronic operon plasmid

   Depositor

   Jeffrey Tabor

   Article

   Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

   Inserts

   ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
   bphP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   insert guanine at position 430 to fix frameshift …

   Promoter

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGRG36-Kn-PA1-GFP

   Plasmid

   #79088

   Purpose

   pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

   Depositor

   Frank Rosenzweig

   Article

   Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

   Insert

   GFP
   

   Use

   Bacterial chromosome integration and expression o…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PA1

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGRB

   Plasmid

   #71539

   Purpose

   for expression of gRNA in E. coli

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J231196

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCC2

   Plasmid

   #128283

   Purpose

   asymmetric plasmid partitioning E. coli - negative control

   Depositor

   Matthew Bennett

   Article

   Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

   Insert

   Piq - mRFP, _parS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+ cMyc-oDam_f_GFPoNLS

   Plasmid

   #85818

   Purpose

   iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   cMyc oDam_f_GFPoNLS
   

   Use

   Tags

   cMyc and oNLS (optimized nuclear localization sig…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A. Codon optimization compatible to work in M…

   Promoter

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-MfnG-EcYRS-EGFP*

   Plasmid

   #191119

   Purpose

   A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-ITR-CMV-mCherry-1xU6-LeuIGI2

   Plasmid

   #217368

   Purpose

   Expresses E. coli leucine tRNA variant "LeuIGI2" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

   Insert

   E. coli leucine tRNA mutant, "LeuIGI2" for TAG suppression
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   C2G, C3G, G6U, A7G, U77C, C78G, G81C, G82C, U83C

   Promoter

   U6

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-iLOVf

    Plasmid

    #63723

    Purpose

    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins

    Depositor

    Brian Smith

    Type

    Empty backbone

    Use

    Tags

    TEE, iLOV, 6xHis, thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a-LIC

    Plasmid

    #62303

    Purpose

    E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOP5GP

    Plasmid

    #112610

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GST and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOP5GT

    Plasmid

    #112611

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GST and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOP5MP

    Plasmid

    #112612

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-MBP and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOP5BT

    Plasmid

    #112609

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GB1 and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Sc RFC5-pCDFDuet

    Plasmid

    #239202

    Purpose

    Overexpress Sc RFC5 in E. coli

    Depositor

    Michael O'Donnell

    Insert

    RFC5 (RFC5 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOP5TT

    Plasmid

    #112613

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-thioredoxin and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOP5BP

    Plasmid

    #112608

    Purpose

    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His-GB1 and Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRF0G-Tyr

    Plasmid

    #113644

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    MjYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pSAH0016

    Plasmid

    #79824

    Purpose

    Dual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicol

    Depositor

    Katja Arndt

    Article

    Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

    Inserts

    cat
    aadA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    AATTCAAAAAAATGCTTGACAATTAATCATCGGCTCGTATAATGTGTGG…

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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