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We narrowed to 18,339 results for: Mut

Showing: 7781 - 7800 of 18339 results

  1. pMCh-646

    Plasmid
    #92289
    Purpose
    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells
    Depositor
    Marina Chekulaeva, Witold Filipowicz
    Article
    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1
    Insert
    dGW182 CED 7W7A (gw Fly)
    Use
    Tags
    NHA
    Expression
    Insect
    Mutation
    dGW182 CED: aa 940-1215 with the following point …
    Promoter
    Ac5
    Available Since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92289-plasmid-map-sequence-id-194379

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  5. pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500

    Plasmid
    #92230
    Purpose
    Expression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    NTerm 6xHis Tagged zmADH
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residue V86 mutated into TAG (Amber) stop codon f…
    Promoter
    p70a
    Available Since
    June 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92230-plasmid-map-sequence-id-181882

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  12. pT22Ubm5En2SRE4PCh(#302)

    Plasmid
    #184089
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    5myc-chkEn2(SR)-ERT2-P2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    5xMyc
    Expression
    Mutation
    chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …
    Promoter
    Zf-Ubi
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184089-plasmid-map-sequence-id-363975

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  13. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid
    #226105
    Purpose
    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    atf4 (ATF4 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains uORF1-2 of ATF4, whose stability can ser…
    Promoter
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226105-plasmid-map-sequence-id-448414

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  14. pET-21b(+)-Is-PETase-W185A

    Plasmid
    #112204
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W185A
    Promoter
    N/A
    Available Since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112204-plasmid-map-sequence-id-215853

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  16. pEVOL-pAzF[TAG]

    Plasmid
    #164579
    Purpose
    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons
    Depositor
    Ryan Mehl
    Article
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    araBAD, glnS, and proK
    Available Since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    164579-plasmid-map-sequence-id-392918

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  19. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

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Showing: 7781 - 7800 of 18339 results