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hCGN(1-406)

Plasmid

#236503

Purpose

For expression of the head domain of CGN (human) in bacteria

Depositor

Sandra Citi

Article

D'Atri et al J Biol Chem. 2002 Aug 2;277(31):27757-64

Insert

CGN (CGN Human)

Tags

GST

Expression

Bacterial

Mutation

WT

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pGlab592

Plasmid

#236554

Purpose

240 basepair upstream sequence of KSHV ORF68 driving HaloTag expression

Depositor

Britt Glaunsinger

Article

Morgens et al Mol Syst Biol. 2025 Jan;21(1):30-58. doi

Insert

KSHV ORF68 upstream 240bp

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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EW434 4x(ZNF35/ZNF35tar)-SFFV-GFP

Plasmid

#236143

Purpose

Fluorescent reporter encoding GFP under control of SFFV promoter with four ZNF35/ZNF35 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

EGFP

Expression

Mammalian

Promoter

SFFV promoter with four ZNF35/ZNF35 binding sites…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW435 4x(ZNF35/IKZF1tar)-SFFV-GFP

Plasmid

#236144

Purpose

Fluorescent reporter encoding GFP under control of SFFV promoter with four ZNF35/IKZF1 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

EGFP

Expression

Mammalian

Promoter

SFFV promoter with four ZNF35/IKZF1 binding sites…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW420 4x(ZNF35/ZNF250tarv3)-SFFV-GFP

Plasmid

#236139

Purpose

Fluorescent reporter encoding GFP under control of SFFV promoter with four ZNF35/ZNF250 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

EGFP

Expression

Mammalian

Promoter

SFFV promoter with four ZNF35/ZNF250 binding site…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pEt28a-SilkTag-CBM-H6

Plasmid

#230903

Purpose

Bacterial expression of SilkTag

Depositor

Sesilja Aranko

Article

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

SilkTag-CBM-H6

Tags

HisTag

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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dimerTomato-wGBD

Plasmid

#129635

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

Cdc42 binding domain of N-WASP

Expression

Mammalian

Promoter

CMVdel

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pJAT22

Plasmid

#204311

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-GFPn

Use

CRISPR

Promoter

3XP3

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX302_circMTO1_OE

Plasmid

#215240

Purpose

Overexpression of pLEX302_circMTO1_OE expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circMTO1

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaAinBCDEOT1T2

Plasmid

#225056

Purpose

Heterologous expression in Streptomyces with inactive SnaA

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaA

Expression

Bacterial

Mutation

S556A/S1616A

Promoter

SP44

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEinOT1T2

Plasmid

#225057

Purpose

Heterologous expression in Streptomyces with inactive SnaE

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaE

Expression

Bacterial

Mutation

deltaA46-V801

Promoter

SP44

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BoxB-petracrRNA

Plasmid

#207625

Purpose

Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

BoxB-petracrRNA

Use

Synthetic Biology

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-BASU-His

Plasmid

#218451

Purpose

E coli expression vector for Halo-BASU-His biotin ligase

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

HaloTag-BASU-His fusion protein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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aTY171_pPGK1_iRFP_WPRE

Plasmid

#225351

Purpose

Lentiviral vector for iRFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

iRFP670

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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aTY173_pPGK1_mTagBFP2_WPRE

Plasmid

#225352

Purpose

Lentiviral vector for BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE-TurboID-HA-rtTA

Plasmid

#226995

Purpose

Backbone plasmid to express TurboID fusion proteins in mammalian cells

Depositor

Bruno Dallagiovanna

Article

Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-Moontag:HA

Plasmid

#219648

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

MoonTag:HA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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sgTelo

Plasmid

#227392

Purpose

Adhesion module, guide RNA recognizes telomeres

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

guide RNA targeting telomeres

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-EYFP:HA

Plasmid

#219656

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

EYFP:HA SEED Cassette

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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p2T_CAG_SpCas9PE2_Igf1-NFATC2IP_BlastR (pepPE2_3)

Plasmid

#173067

Purpose

Expresses NI-PE2

Depositor

Richard Sherwood

Article

Velimirovic et al Nat Commun. 2022 Jun 18;13(1):3512. doi:

Insert

Igf1-NFATC2IP

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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