Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 15,155 results for: pET

Clear keywords
Showing: 7841 - 7860 of 15155 results

  5. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid
    #153445
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available Since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153445-plasmid-map-sequence-id-306484

    Enlarge

  6. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid
    #153444
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available Since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153444-plasmid-map-sequence-id-306456

    Enlarge

  14. pJC2747 - pET28-SKP1(A2P, ∆38-43, ∆71-82)-FBXO31(∆1-65, D334N)

    Plasmid
    #250278
    Purpose
    Recombinant production of SKP1(A2P, ∆38-43, ∆71-82) and 6His-Smt3-StrepII-FBXO31(∆1-65, D334N).
    Depositor
    Jacob Corn
    Article
    Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:
    Inserts
    SKP1 (SKP1 Human)
    FBXO31 (FBXO31 Human)
    Tags
    6xHis-Smt3(SUMO)-StrepII
    Expression
    Bacterial
    Mutation
    A2P, ∆38-43, ∆71-82 and ∆1-65, D334N
    Promoter
    T7
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250278-plasmid-map-sequence-id-498555

    Enlarge

  16. pET28-His6-SUMO-Lyn-SH2-AviTag

    Plasmid
    #214207
    Purpose
    Bacterial expression of SH2 domain of the Lyn kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Lyn A (LYN Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214207-plasmid-map-sequence-id-422791

    Enlarge

  17. pET28-His6-SUMO-Yes-SH2-AviTag

    Plasmid
    #214208
    Purpose
    Bacterial expression of SH2 domain of the Yes kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Yes kinase SH2 domain (YES1 Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214208-plasmid-map-sequence-id-422836

    Enlarge

  18. pET28-His6-SUMO-Lck-SH2-AviTag

    Plasmid
    #214203
    Purpose
    Bacterial expression of SH2 domain of the Lck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Lck kinase SH2 domain (LCK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214203-plasmid-map-sequence-id-422773

    Enlarge

  19. pET28-His6-SUMO-Blk-SH2-AviTag

    Plasmid
    #214204
    Purpose
    Bacterial expression of SH2 domain of the Blk kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Blk kinase SH2 domain (BLK Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214204-plasmid-map-sequence-id-422774

    Enlarge

  20. pET28-His6-SUMO-Fgr-SH2-AviTag

    Plasmid
    #214205
    Purpose
    Bacterial expression of SH2 domain of the Fgr kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Article
    Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi
    Insert
    Fgr kinase SH2 domain (FGR Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    214205-plasmid-map-sequence-id-422780

    Enlarge

Showing: 7841 - 7860 of 15155 results