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sgLacZs

Plasmid

#171185

Purpose

Expresses sgRNA

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgLacZ1-hU6-sgLacZ2

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGC(2.1)-U6.3

Plasmid

#170516

Purpose

PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Insert

gRNAcore(2.1)-pU6.3

Use

Pcr template vector for amplifying grnacore(2.1)-…

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC2-GPT2-3xFLAG

Plasmid

#163447

Purpose

lentiviral overexpression vector for human GPT2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GPT2 (GPT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

Plasmid

#128404

Purpose

Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Exotoxin A (toxA P. aeruginosa)

Tags

6xHis tag and DsbA signal sequence for periplasmi…

Expression

Bacterial

Mutation

242DNNNS246 and 384DQNRT388 engineered glycosylat…

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry-MKLP1

Plasmid

#140562

Purpose

Expresses mCherry tagged MKLP1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

MKLP1 (KIF23 Human)

Tags

2xmCherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

Plasmid

#124864

Purpose

Mutagenesis of Grin2b

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2b (Grin2b Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC deltab_P2A_Hygro_Barcode

Plasmid

#120507

Purpose

Barcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC deltab (MYC Human)

Use

Lentiviral

Mutation

Deletion of basic motif

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSirenRetroQ(puro)-shEgr3

Plasmid

#108115

Purpose

pSIREN-RetroQ vector containing shRNA sequence to Egr3

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

shRNA to Egr3 (Egr3 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-kanMX6

Plasmid

#105160

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-kanMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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OXSM_pLX307

Plasmid

#98357

Purpose

Lentiviral expression of OXSM

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

OXSM (OXSM Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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ADSS_pLX307

Plasmid

#98316

Purpose

Lentiviral expression of ADSS

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ADSS (ADSS2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pH3FS-PYK1

Plasmid

#85781

Purpose

N-ICE plasmid pH3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(T308A/S473A)

Plasmid

#86578

Purpose

Expresses human full-length Akt1(T308A/S473A) phosphomutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

T308A/S473A phosphomutant

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-human MYCN-V421D

Plasmid

#74166

Purpose

Mammalian expression of human MycN-VD mutant

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

MycN (MYCN Human)

Tags

HA

Expression

Mammalian

Mutation

V421D

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-mouse c-MycV394D-IRES-RFP

Plasmid

#73573

Purpose

Mammalian retroviral expression of mouse Myc V394D mutant

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMyc (Myc Mouse)

Use

Retroviral

Tags

IRES-RFP

Expression

Mammalian

Mutation

V394D

Promoter

MSCV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
p3A9cbetaTCR.HSV2.3

Plasmid

#69594

Purpose

TCR beta chain genomic transgenic construct for generation of gBT-I mice

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR beta VJ region from clone HSV2.3 (Tcrb Mouse)

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
JA1246

Plasmid

#49960

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD HB-5 (HBB Human)

Use

TALEN

Promoter

EF1a

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only
pAC1-pCR8-dCas9VP48

Plasmid

#48214

Purpose

dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP48 activation domain

Use

CRISPR and Gateway Cloning

Tags

HA Tag and VP48

Mutation

D10A;H840A

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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Hox A13a

Plasmid

#36463

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxA13a (hoxa13a Zebrafish)

Use

Riboprobe synthesis

Mutation

contains aa102-290; M107T

Promoter

T7

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N281

Plasmid

#17524

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 281 amino acids

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only