We narrowed to 29,789 results for: puromycin

Showing: 7861 - 7880 of 29789 results

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pCIP-Flag-TfebAA

Plasmid

#79014

Purpose

For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Tfeb (Tfeb Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S142A, S211A

Promoter

pCAGGs

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-PML-IV

Plasmid

#62804

Purpose

expresses the isoform IV of PML (promyelocytic leukemia protein)

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

PML-IV (PML Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only
MSCV-(mcs) human CSF1 full length-IRES-Puro

Plasmid

#86797

Purpose

retroviral expression of CSF-1

Depositor

Martine Roussel, Charles Sherr

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

CSF-1 (CSF1 Human)

Use

Retroviral

Tags

IRES-Puro

Expression

Mammalian

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-FlaviA-GFP-puro

Plasmid

#140088

Purpose

Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

Insert

FlaviA-GFP

Use

Lentiviral; Low expression

Tags

6XHis tag and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176273

Purpose

Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (sgTet) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-HsSAS6

Plasmid

#136790

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-MITF

Plasmid

#64888

Purpose

Retroviral expression of murine MITF

Depositor

Ivana de la Serna

Article

de la Serna et al J Biol Chem. 2006 Jul 21;281(29):20233-4

Insert

MITF (Mitf Mouse)

Use

Retroviral

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
CROP-sgRNA-MS2

Plasmid

#153457

Purpose

CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold

Depositor

Wolf Reik

Article

Alda-Catalinas et al Cell Syst. 2020 Jul 22;11(1):25-41.e9. d

Insert

sgRNA empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-ZIKVA-GFP-puro

Plasmid

#140090

Purpose

Fluorescence activatable reporter of ZIKV NS2B-NS3 protease activity

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

Insert

ZIKVA-GFP

Use

Lentiviral; Low expression

Tags

6XHis tag and GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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piggyBAC-H2B FR-MQV

Plasmid

#194079

Purpose

Expresses nuclear-localized FR-MQV in mammalian cells

Depositor

Ralph Jimenez

Article

Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

Insert

FR-MQV

Tags

Histone H2B

Expression

Mammalian

Mutation

Methionine 42 to Glutamine, Cystiene 159 to Valin…

Promoter

CMV7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLPCX-VEcadTS

Plasmid

#45848

Depositor

Martin Schwartz

Article

Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

Insert

VE-cadherin Tension Sensor (Cdh5 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Sox10

Plasmid

#46536

Purpose

A lentiviral vector for the expression of mouse Sox10 under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Sox10 (Sox10 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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dCas9-MSK1

Plasmid

#165601

Purpose

Expresses Sp dCas9 fused to human MSK1

Depositor

Isaac Hilton

Article

Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

Insert

ribosomal protein S6 kinase A5 (RPS6KA5 S. Pyogenes, Synthetic, Human)

Use

CRISPR and Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Promoter

EF1a

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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hMLKL-Venus

Plasmid

#106078

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

human MLKL-Venus (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

Venus

Expression

Mammalian

Promoter

Dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-ERM

Plasmid

#199664

Purpose

expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

SEC61B and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

Plasmid

#118617

Purpose

Lentiviral expression of mTurq2 tagged PCNA

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

TK-NLS-mTurq2-PCNA (PCNA Human)

Use

Lentiviral

Tags

mTurq2

Expression

Mammalian

Promoter

hPGK

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Puro-moxGFP

Plasmid

#227275

Purpose

AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE FLAG-IKK2-EE puro

Plasmid

#216790

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Kevin Janes

Article

Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

Insert

IKK-2 (IKBKB Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Promoter

LTR

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCrispr-V2-mOrange (V2mO)

Plasmid

#140206

Purpose

Lenti Crispr/Cas9 with mOrange fluorescent marker

Depositor

Jaffer Ajani

Article

Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

pELF1a

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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