We narrowed to 20,117 results for: Cre

1. pLib6.4B-Consensus

   Plasmid

   #176678

   Purpose

   Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28C-mCherry-YTHDF2

   Plasmid

   #204406

   Purpose

   E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGWB545

   Plasmid

   #74879

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAT9658-sgRNA-mCherry

   Plasmid

   #162987

   Purpose

   sgRNA cloning plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LB701: pMVP/PB/mCherry-DEST

   Plasmid

   #121871

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVk7-33G1-Flag

   Plasmid

   #58950

   Purpose

   backbone for mouse recombinant antibody with added C-terminal FLAG tag

   Depositor

   Gavin Wright

   Article

   Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

   Type

   Empty backbone

   Tags

   FLAG tag (DYKDDDDK)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB521

   Plasmid

   #74863

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. IY601: pMVP/Lenti/Blast-DEST

   Plasmid

   #121849

   Purpose

   pMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Lentiviral; Pmvp gateway destination vector

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAT9650-BEAR-GFP-preedited

   Plasmid

   #162992

   Purpose

   BEAR control plasmid with split EGFP and intact 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LC301: pMVP/PB/Zeo-DEST

    Plasmid

    #121877

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PBMN YFP-ER50

    Plasmid

    #37261

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al J Am Chem Soc. 2012 Mar 7;134(9):3942-5.

    Insert

    ER50 (ESR1 Human)
    

    Use

    Retroviral

    Tags

    HA-YFP

    Mutation

    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

    Promoter

    LTR

    Available Since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSIIbsr-6013x

    Plasmid

    #108654

    Purpose

    An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    Raichu-EV
    

    Use

    Lentiviral

    Tags

    XFP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)

    Plasmid

    #213034

    Purpose

    fusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Slaviero et al Neurophotonics. 2024 Apr;11(2):024208. d

    Insert

    mNeonGreen, ISN-IE, ChRmine
    

    Use

    AAV

    Tags

    Gaussia luciferase signal sequence

    Promoter

    hSyn

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM232

    Plasmid

    #226654

    Purpose

    pET24b-p97-FLAG

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Tags

    FLAG

    Expression

    Bacterial

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGWB520

    Plasmid

    #74862

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. LD101: pMVP (L3-L2) eCFP + WPRE

    Plasmid

    #121775

    Purpose

    pMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eCFP + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB410

    Plasmid

    #74804

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AlkB-D135T

    Plasmid

    #160433

    Purpose

    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

    Depositor

    Tao Pan

    Article

    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

    Insert

    E. coli AlkB
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 135 to Threonine; deleted t…

    Promoter

    T7

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_085

    Plasmid

    #158583

    Purpose

    Lentiviral expression of SpCas9; P2A site provides mKate2

    Depositor

    John Doench, David Root

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Insert

    SpCas9
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVk7-33G1-BioLHis

    Plasmid

    #58949

    Purpose

    backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

    Depositor

    Gavin Wright

    Article

    Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

    Type

    Empty backbone

    Tags

    6His tag and biotinylation peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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