We narrowed to 20,117 results for: Cre

1. pET28C-mCherry-SARS-CoV-2-Nucleocapsid

   Plasmid

   #204403

   Purpose

   E. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
   

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGLucIPZ

   Plasmid

   #228703

   Purpose

   Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

   Depositor

   Thorsten Stiewe

   Article

   Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

   Inserts

   Gaussia luciferase
   non-silencing shRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 2, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. EF1a_SPIB_P2A_Hygro_Barcode

   Plasmid

   #120483

   Purpose

   Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SPIB (SPIB Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-U6sgbbBsmbI-puro-2A-Fluc

   Plasmid

   #100277

   Purpose

   Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB420

   Plasmid

   #74814

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB508

   Plasmid

   #74850

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-sgKmt2d-sg1

   Plasmid

   #192166

   Purpose

   Lenti-sgKmt2d-sg1

   Depositor

   Sidi Chen

   Article

   Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

   Type

   Empty backbone

   Use

   Lentiviral

   Mutation

   NA

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG--uMASS_LF

   Plasmid

   #209763

   Purpose

   Expression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoter

   Depositor

   Andre Berndt

   Article

   Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

   Insert

   uMASS_LF
   

   Expression

   Mammalian

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-uMASS_1

   Plasmid

   #209762

   Purpose

   Expression of uMASS_1 in mammalian cells under CAG promoter

   Depositor

   Andre Berndt

   Article

   Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

   Insert

   uMASS_1
   

   Expression

   Mammalian

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. ST-SBP-mCherry_puromycin

    Plasmid

    #65271

    Purpose

    ST reporter only ( no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. NylB'-SCY_pET21b

    Plasmid

    #215417

    Purpose

    Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'-SCY
    

    Expression

    Bacterial

    Mutation

    R187S/F264C/D370Y

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLib6.4-Aalb_743

    Plasmid

    #176662

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBK-YE2-BE3

    Plasmid

    #85176

    Purpose

    Expresses YE2-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YEE-BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYSD300

    Plasmid

    #212930

    Purpose

    Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    AGA1 (AGA1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG306-GPD-VMA1 chr I

    Plasmid

    #41896

    Purpose

    Yeast expression of VMA1.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    VMA1 (VMA1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Contains silent mutation at 342A-G and L87S mutat…

    Promoter

    GPD

    Available Since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI pHluorin Syt11

    Plasmid

    #184512

    Purpose

    Mammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 11 (Syt11 Mouse)
    

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. actc1b-mKate2-ING2

    Plasmid

    #109502

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-ING2
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL3.7-K122-TEAD-p-H2B-mCherry

    Plasmid

    #139648

    Purpose

    Reporter assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    IRES-GFP
    

    Use

    Lentiviral

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-BFP

    Plasmid

    #106282

    Purpose

    A version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    CMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-Decoy

    Plasmid

    #80324

    Purpose

    Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    Decoy sgRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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