We narrowed to 62,316 results for: cloning
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Plasmid#242606PurposeA CAGGS driven expression vector containing an actin nanobody fused to sfGFP to label actin followed by a HA tagged tdiRFP fused to a caax tag to label the cell membrane.DepositorInsertActin Vhh-sfGFP-P2A-HA-tdiRFP-caax
ExpressionMammalianPromoterCAGGSAvailable SinceDec. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)
Plasmid#229810PurposeA Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.DepositorInsertloxp-AmCyan-stop-lox
UseTol2 based expression vectorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tet-Off-FLEX-DEST (JDW 1186)
Plasmid#229820PurposeAn AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTADepositorTypeEmpty backboneUseAAVAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)
Plasmid#229826PurposeTet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)DepositorInsertmScarlet-I
UseAAVAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)
Plasmid#229839PurposeA CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.DepositorInsertLuciferase
ExpressionMammalianAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX20
Plasmid#169443PurposeUsed for guide cloning with PCRDepositorTypeEmpty backboneUseCRISPRAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-T308A
Plasmid#49189PurposecDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalMutationT308APromoterT7Available SinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pICSL41308-LC
Plasmid#245696PurposeLevel 0 Golden Gate Coding Sequence (WITH stop codon) acceptor, Low - copy with pBR322 origin of replication AATG - GCTTDepositorTypeEmpty backboneUseSynthetic BiologyMutationBsaI/BpiI sites removed by point-mutationAvailable SinceDec. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pICSL01005_CP
Plasmid#245689PurposeLevel 0 Golden Gate Coding Sequence (WITHOUT stop codon) acceptor with AmilCP negative selection cassette AATG - TTCGDepositorTypeEmpty backboneUseSynthetic BiologyMutationBsaI/BpiI sites removed by point-mutationAvailable SinceDec. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pICSL60027
Plasmid#245675PurposeLevel 0 Golden Gate part Extensin-Truncated-EU + NbACT + Rb7MAR (D144-437 variant) terminator, GCTT - CGCTDepositorInsertExtensin-Truncated-EU + NbACT + Rb7MAR (D144-437 variant) terminator
UseSynthetic BiologyMutationBsaI/BpiI sites removed by point-mutationAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-PPP2CB-C-HA
Plasmid#195179PurposeVector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRU46
Plasmid#167668PurposeENTRY vector for gRNA6 cloning under pAtU3 promoterDepositorInsertpAtU3
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-3xP3-RFP
Plasmid#130276PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.DepositorInsert3xP3-RFP
UseCRISPRExpressionInsectAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDSRaD-sdi1
Plasmid#219566PurposeMammalian expression of human sdi1 (p21 CDK inhibitor)DepositorAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM033_P7_3"His5
Plasmid#216458Purpose3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.DepositorInsert3''his5
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM026_P2_pNMT1
Plasmid#216451PurposeRegulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter nmt1
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM019_P2_pRPL2102
Plasmid#216444PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter rpl2102
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM015_P2_pADH1
Plasmid#216440PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter adh1
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM013_P2_pENO101
Plasmid#216438PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter eno101
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTB3-Myc tag
Plasmid#210205PurposeType 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.DepositorInsertMyc-tag
ExpressionBacterialAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only