We narrowed to 9,204 results for: sgRNA

Showing: 7901 - 7920 of 9204 results

Results per page:

Sort results by:

pSCL452

Plasmid

#219502

Purpose

Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Depositor

Seth Shipman

Publication

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Expression

Yeast

Promoter

GAL7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Sha3Cas9

Plasmid

#192136

Purpose

Expresses Sha3Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Publication

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sha3Cas9

Use

AAV

Expression

Mammalian

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pspCas9-3exo-Tetra-com-CLCN5-sp-g1

Plasmid

#176236

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Publication

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176237

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Publication

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
sgAAT Nicking

Plasmid

#169842

Purpose

Express a nicking sgRNA used for correction (via A•T-to-G•C) of the E342K mutation

Depositor

Wen Xue

Publication

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

sgAAT Nicking (SERPINA1 Human)

Expression

Mammalian

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 CARD8 PAM

Plasmid

#169975

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Publication

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

PAM

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
LentiCRISPR v2-sgAMPK alpha2 clone2

Plasmid

#162123

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Publication

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR v2-sgAMPK alpha2 clone3

Plasmid

#162124

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Publication

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYMCR2_Gb_PMLTSS

Plasmid

#140285

Purpose

CRISPR/Cas9 plasmids encoding sgRNA targeting TSS of PML for knock-in

Depositor

Yusuke Miyanari

Publication

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

Cas9

Expression

Mammalian

Promoter

CBH

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pX300A-G+Nanog

Plasmid

#140280

Purpose

CRISPR/Cas9 plasmid encoding Cas9 and sgRNA against gBait and Nanog locus

Depositor

Yusuke Miyanari

Publication

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

Cas9

Expression

Mammalian

Promoter

CBH

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ141-ZmUbi-RZ-Bth

Plasmid

#129686

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Publication

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty Backbone

Use

CRISPR

Expression

Plant

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCMV-Blast mLST8-Flag Mut1

Plasmid

#124916

Purpose

For stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L mutant

Depositor

Jin Chen

Publication

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCMV-Blast mLST8-Flag Mut2

Plasmid

#124917

Purpose

For stable expression_ sgRNA resistant Flag tagging mLST8 W272F/W274F mutant

Depositor

Jin Chen

Publication

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MLST8 (MLST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPN067

Plasmid

#91602

Purpose

Express sgRNA targeting human DPP4

Depositor

Lindy Barrett

Publication

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DPP4 (DPP4 Human)

Use

CRISPR

Promoter

U6

Available since

Oct. 12, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pPN088

Plasmid

#91613

Purpose

Express sgRNA targeting human FURIN

Depositor

Lindy Barrett

Publication

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

FURIN (FURIN Human)

Use

CRISPR

Promoter

U6

Available since

Oct. 12, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCas28

Plasmid

#82385

Purpose

sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23108

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas27

Plasmid

#82384

Purpose

sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Publication

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PJ23117

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DNA repair library with 336 sgRNAs

Pooled library

#177664

Purpose

CRISPR-interference (CRISPRi) library of 366 sgRNAs targeting 118 genes encoding factors involved in DNA repair and associated processes

Depositor

Brittany Adamson

Publication

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Species

Homo sapiens

Use

CRISPR

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only
mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP

Pooled library

#197349

Purpose

To allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.

Depositor

Rebecca Voorhees, Jonathan Weissman

Publication

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Species

Homo sapiens

Use

Lentiviral

Available since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

Plasmid

#223321

Purpose

It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.

Depositor

Jonathan Arias

Publication

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Inserts

SpCas9 codon optimized for human (cas9 Human, Synthetic)
chloramphenicol resistance protein
CcdB

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CBh

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge