We narrowed to 14,429 results for: nts
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(559) pAAV EF-1a KRAB-SadCas9 U6-gRNA
Plasmid#163024PurposeExpression of CRISPRi with U6-gRNA (Bsa1 sites)DepositorInsertMammalian codon-optimized SadCas9
UseAAVTagsKRAB, SV40 polyA, and myc NLSExpressionMammalianMutationPromoterhEF-1aAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso1
Plasmid#159585PurposeExpress N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cellsDepositorInsertDHX36 (DHX36 Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luc-T20
Plasmid#177941PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP71-tCD19-mGFP
Plasmid#174609Purposeexpresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19DepositorInsertCD19 extracellular and transmembrane domain (Cd19 Mouse)
UseTagspalmitoylated EGFPExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
FusionRed-Fascin-C-10
Plasmid#56117PurposeLocalization: Cytoskeleton, Excitation: 580, Emission: 608DepositorInsertFascin (FSCN1 Human)
UseTagsFusionRedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EFS-DIO-GRAB_OT1.0
Plasmid#185387PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin sensor GRAB_OT1.0
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BTB+BACK)
Plasmid#169723PurposeMammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)DepositorInsertSPOP (Spop Mouse)
UseTagsV5ExpressionMammalianMutationL186D, L190D, L193D, I217K, Y353EPromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luc-PS9
Plasmid#177942PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-Halo7Tag-hFis
Plasmid#111136PurposehFis with selflabeling tag, e.g. for fluorescence microscopyDepositorInserthuman fission factor1 (FIS1 Human)
UseTagsHalo7-tagExpressionMammalianMutation*PromoterCMVAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MPRA-MluI-SpeI-EcoRI
Plasmid#190196PurposeEmpty vector for inserting MPRA libraries into AAVDepositorTypeEmpty backboneUseAAV; Massively parallel reporter assay (mpra)TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-88)-Strep
Plasmid#196214PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCerulean-cMyc
Plasmid#23244DepositorInsertmCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-T308D/S473D
Plasmid#49192PurposecDNA of Akt1 with myristyolation signal and point mutation from T to D at position 308 and S to D at position 473 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationT308D; S473DPromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCherry-Klf4
Plasmid#23243DepositorInsertmCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCK341
Plasmid#192640PurposeExpresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dSpRY
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyTagsExpressionMutationSoxS has R93A and S101A mutations and dSpRY has s…PromoterBBa_J23107 and Sp.pCas9Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHSN401
Plasmid#50588PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInserts3×FLAG-NLS-zCas9-NLS
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG and NLSExpressionMutationmaize codon optimizedPromoter2×35Sp and AtU6-26pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_PTEN(C124S)-P2A-Hygro_Barcode
Plasmid#170248PurposeBarcoded lentiviral vector to express PTEN (C124S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertPTEN (PTEN Human)
UseLentiviralTagsExpressionMutationC124SPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGreenFire_blastocidin
Plasmid#174103PurposepGreenFire MPRA plasmid for cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST ERKKTRClover
Plasmid#90227PurposeLentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCloverExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only
