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OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast

Plasmid

#128010

Purpose

traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

Use

Drosophila osc reporter construct

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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mTFP1-Clathrin-15

Plasmid

#55471

Purpose

Localization: Clathrin Vesicles, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Clathrin (CLTB Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pDK637

Plasmid

#134203

Purpose

bacterial expression of human CENP-N 1-240

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N 1-240-His (CENPN Human)

Expression

Bacterial

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

Plasmid

#192160

Purpose

Dual AAV vector AAV-dCas9

Depositor

Sidi Chen

Article

Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

Insert

Dead Cas9

Use

AAV

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pQTEV-MAP1LC3A

Plasmid

#34824

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pTag-RFP-C-h-Rab4a-c-Myc

Plasmid

#79800

Purpose

Express TagRFP labeled human Rab5a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab4a (RAB4A Human)

Tags

TagRFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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mTFP1-Alpha-V-Integrin-25

Plasmid

#55465

Purpose

Localization: Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Alpha-V Integrin (ITGAV Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENeGFP PCNAL2 pc653

Plasmid

#167564

Purpose

The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

Depositor

Cristina Cardoso

Article

Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp13

Plasmid

#157713

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp13 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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p415TEF cyto roGFP(iL)-Grx1(human)

Plasmid

#65002

Purpose

Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP(iL)

Expression

Yeast

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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Beta2-AR-TYY

Plasmid

#32694

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2006 Jan 13;281(2):1261-73.

Insert

β2AR (ADRB2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

T68F, Y132G Y219A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pEX-CMV-SP-YFP-STM2(15-746) EF hand D80A

Plasmid

#18864

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal interaction molecule 2 (STIM2 Human)

Tags

YFP

Expression

Mammalian

Mutation

EF hand mutant: D80A. YFP inserted between signal…

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pF153 pcDNA3.1(+)SOD1G37R

Plasmid

#26399

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

G37R

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nsp12-HF

Plasmid

#157711

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.3−3xFLAG−AGO2(S387D)

Plasmid

#220463

Purpose

Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Serine 387 to aspartate

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-GFP-RELA K310R

Plasmid

#196630

Purpose

Express GFP-RELA K310R

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

GFP-RELA K310R (RELA Human)

Use

Lentiviral

Tags

GFP

Mutation

K310R

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-hPCM2w/b delta6

Plasmid

#47588

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b delta6 (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-S305A-3xFLAG

Plasmid

#69839

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305A

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pGEMHE-NLS-Beta-catenin-S33Y

Plasmid

#138537

Purpose

For making Beta-catenin-S33Y RNA

Depositor

Carmen Williams

Article

Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

Insert

Beta-catenin

Use

In vitro transcription

Tags

2XFLAG and 2xNLS

Mutation

changed serine 33 to tyrosine

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-JAM-A

Plasmid

#112965

Purpose

To express feline Jam-A transiently in cell lines that do not naturally express the receptor.

Depositor

John S. L. Parker

Article

Lu et al J Virol. 2018 Mar 28;92(8):e00035-18. do

Insert

Junctional Adhesion Molecule type A (JAM-A) (F11R Felis catus)

Expression

Mammalian

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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