We narrowed to 14,335 results for: ung

Showing: 7941 - 7960 of 14335 results

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#1.4 post-G-SynapShot-mScarlet

Plasmid

#214716

Purpose

Expresses green #1.4 post-SynapShot and mScarlet

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 post-G-SynapShot

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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#1.4 pre-G-SynapShot-TagBFP

Plasmid

#214717

Purpose

Expresses green #1.4 pre-SynapShot and TagBFP

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 pre-G-SynapShot

Use

AAV

Tags

TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_206

Plasmid

#96920

Purpose

for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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PX333-T2A-EGFP

Plasmid

#197417

Purpose

SpCas9-T2A-EGFP with dual sgRNA cloning backbones

Depositor

Yeh-Hsing Lao

Article

Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CBh; U6

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b-RvLEAMshort

Plasmid

#218122

Purpose

Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

Depositor

Ci Ji Lim

Article

Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

Insert

RvLEAMshort

Tags

6xHIS

Expression

Bacterial

Mutation

Truncated form (58-181aa)

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-NRF1

Plasmid

#34707

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pLenti_dCas9-KRAB-MeCP2_Blast

Plasmid

#221501

Purpose

Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

dCas9-KRAB-MeCP2-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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HBV 1.3-mer X-null replicon

Plasmid

#65461

Purpose

containing 1.3 units of the X-null HBV genome (subtype ayw)

Depositor

Wang-Shick Ryu

Article

Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

Insert

1.3 units of the X-null HBV genome

Expression

Mammalian

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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LifeAct-GFP-cytoB5RR

Plasmid

#182577

Purpose

Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

LifeAct-GFP-cytoB5RR

Tags

LifeAct and cytoB5RR

Expression

Mammalian

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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UbG76V-eGFP-V5His_20aa

Plasmid

#23970

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_20aa

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Available Since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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pMCSG53-DsbC

Plasmid

#186623

Purpose

E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Tags

DsbC-6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pX330_human CDK1

Plasmid

#118597

Purpose

One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

guide RNA against human CDK1

Use

CRISPR

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pM378

Plasmid

#173174

Purpose

Expresses C9-1 gRNA-12xMBS from hU6 promoter

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

C9-1 targeting gRNA-12xMBS

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pM355

Plasmid

#173175

Purpose

(Empty Backbone) Expresses gRNA-12xMBS from hU6 promoter

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

gRNA-12xMBS backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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#2.4 pre-G-SynapShot-TagBFP

Plasmid

#214721

Purpose

Expresses green #2.4 pre-SynapShot and TagBFP

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#2.4 pre-G-SynapShot

Use

AAV

Tags

TagBFP

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLiLucB

Plasmid

#31596

Depositor

Michele Calos

Article

Woodard et al PLoS One. 2010 . 5(6):e11367.

Insert

alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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piggyBAC-H2B mCherry-XL (R125K)

Plasmid

#189926

Purpose

Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

Depositor

Ralph Jimenez

Article

Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

Insert

mCherry-XL (R125K)

Tags

H2B

Expression

Mammalian

Mutation

differs from mCherry by R125K, W143S, I161V, Q163…

Promoter

CMV7

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Fis1

Plasmid

#182580

Purpose

Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

mCherry-Fis1

Tags

Fis1

Expression

Mammalian

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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PL-SIN-Oct4-EGFP

Plasmid

#21319

Purpose

Lentiviral vector, expresses EGFP under Oct3/4 promoter

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

Oct-4 promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pEF_dCas9-VP64

Plasmid

#68417

Purpose

Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

dCas9

Use

CRISPR

Tags

3xFLAG and VP64

Expression

Mammalian

Mutation

D10A/H841A

Promoter

human EF1[alpha]

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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