...informative events are one of the main ways budding young scholars across Europe get introduced to the cultures...organisation is to encourage iGEM alumni to mentor younger students in society and to support iGEM alumni-...and networking meetups. The society hopes to help young scientists interested in this field excel and develop...to see these biological societies engaging with young scholars. We believe it’s very important to develop...mentioned above provide strong foundations upon which young scientists can build powerful networks and we encourage...

... Plasmid Collections Synthetic Biology Fungal Synthetic Biology: Fungal SynBio Resources...collection of synthetic biology plasmids for use in fungus. Fungal Plasmids Search the table by keyword or sort... for use in fungus. Plasmid...

...Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids... Genome Engineering CRISPR Joung Lab CRISPR Plasmids...Plasmids Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids You may also like... CRISPR Guide CRISPR... endonuclease) that cleaves the target DNA. The Joung lab recently described gRNA and Cas9 expression ...Hwang and Fu et al., Nat Biotechnol. 2013). The Joung lab has also modified their web-based ZiFiT Targeter...newsgroup here . Links to additional pages describing Joung Lab CRISPR-Cas/RGN reagents: Cpf1 expression plasmids...

...uracil N-glycosylase (UNG) during base excision repair. Adding a second copy of the UNG inhibitor, UGI, increases...and cytosine editing components together. Keith Joung’s lab created a dual-deaminase editor called SPACE...Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G (2020) Directed evolution of adenine...Iyer S, Miller BR, Langner LM, Hsu JY, Aryee MJ, Joung JK (2020) A dual-deaminase CRISPR base editor enables...Wyvekens N, Khayter C, Iafrate AJ, Le LP, Aryee MJ, Joung JK (2014) GUIDE-seq enables genome-wide profiling...

...pipette ready, push down the plunger. There will be multiple “stops” on the plunger, where you feel resistance... front view photo of a P1000 pipette shows the plunger at the top, which is used to draw and expel liquid...shows the volume adjustment ring right below the plunger. This ring changes the pipette volume. Below that...behind the numerical display or the top of the plunger of the pipette. Select the pipette tip that is ...ve reached step 8. Continuing to hold down the plunger, gently lower the pipette tip into the liquid. ...end of the tip is submerged, slowly release the plunger, allowing it to go back to its resting position...the pipette tip). Then, slowly press down on the plunger all the way to the second stop to release the liquid...

...Disinfectant may be added to prevent growth of bacteria or fungi. There are disinfectants designed to be used in ...

...to New England Biolabs by TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO....

...explains. Elizabeth Unger: Developing a serotonin sensor The goal of Elizabeth Unger’s postdoctoral work...Commemorative Travel Awards: Melissa Chiasson, Elizabeth Unger, and Michal Bernach. Melissa Chiasson: Quantifying... fluorescent sensor for serotonin. According to Unger, “serotonin is a well-known but poorly studied neuromodulator...incredibly important for nearly every aspect of life.” Unger began with the a bacterial periplasmic binding protein...no known bacterial proteins that bind serotonin. Unger redesigned the binding pocket of the periplasmic...for serotonin by over four orders of magnitude. Unger validated the serotonin sensor with proof-of-principle...can also kinetically outperform current methods. Unger plans to use the sensor to answer the question of...

... on the lungs, and, among other projects, using stem cells to develop in vitro models for lung disease...goal - to develop therapies to treat disease in the lung. Chapter 2: Balancing research with medical practice...used to better understand the biology of certain lung diseases. Chapter 6: Near-term possibilities for...for using engineered cells in therapy. The lungs are difficult, but other tissues like the retina have ...therapies underway. Chapter 7: Generating entire lungs using a decellurized scaffold as a base. Darrell... using stem cells from patients to study various lung diseases. Chapter 10: How Darrel defines Open Science...

...mechanism unknown)   Chen et al., 2023 AAV9.452sub.LUNG1 Lung: ATII cells Mice N/A Goertsen et...type II cell transduction in the rodent lung AAV9.452sub.LUNG1 is an engineered capsid derived from AAV9...has tropism for lung cells, and has increased overall transduction throughout the lungs. Like other engineered...AAV9 that effectively transduces mouse lungs, targeting alveolar epithelial type II cells (Goertsen, Goeden... does express in the brain. Both AAV9 and AAV9.452sub.LUNG1 have significantly higher off-target expression...including PAL1D, are detargeted from the liver, kidney, lung, and thymus, and transduce the heart and spleen ...Flytzanis, N. C., & Gradinaru, V. (2022). Targeting the lung epithelium after intravenous delivery by directed...

...Cas9s with novel PAM recognition In 2015, Keith Joung’s lab performed a series of positive selection screens...Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev...Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing with CRISPR-Cas13....Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim J-S (2017) In vivo...Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered CRISPR-Cas9 nucleases with ...Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK (2016) Genome-wide specificities of CRISPR-Cas...Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F (...

...to their own devices. Chytrid fungi (Lillian Fritz-Laylin) Chytrid fungi are useful for the study of basic... basic evolution of fungi and eukaryotes and includes the fungus that is killing frogs all over the world...diverse plants, animals, microbes, viruses, and fungi for which these methods are presently unavailable...world. These are less well known fungi (they are not yeast or mushrooms) but there are over 1,000 species...

...References Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT, Shmakov S, Makarova KS... Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA,..., Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic...science.aam9321 Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable...Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV ...Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F (...

...possible TF-induced cellular states was generated (Joung et al., 2023). Fig. 1: Barcoded TF library...performed to map TFs to cell fates. (Adapted from Joung et al.)    This atlas linked TF expression to... nominated reference cell types.  (Adapted from Joung et al.)    Putting MORF to work Screens and ...addgene.org! References and Resources  References Joung J. et al., A transcription factor atlas of directed...186 (1) 209-229. doi: 10.1016/j.cell.2022.11.026 Joung J. et al., Genome-scale CRISPR Cas-9 knockout and...

...//doi.org/10.3390/cells8121510 Sung H, Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based...is quick, efficient, reliable and cost-effective (Sung, 2006). The sensor cell-based method uses specialized... mycoplasmas but also avoid other bacterial and fungal contamination. Proper PPE Since humans are the ... changed regularly as mycoplasma, bacteria, and fungus can reside in these containers. Clean up any media...

...stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their behavior. ...point where the temperature and humidity favors fungal growth. The ants bites down and locks their jaws...its muscles atrophy. After the ant’s death, the fungal fruiting bodies being to sprout out of the ant’...it out of hiding to become exposed to predators. Hungry birds spots the “caterpillar” and then poke out...

...variants derived from analysis of lung adenocarcinoma genomes (Lung Cancer Mutant Collection) or “pan-...variants and genes: High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, Brooks AN, .... Cancer Cell, 2016. PubMed PMID: 27478040. The lung cancer mutant collection is described in this paper...

...Zhang Lab had described eSpCas9, while the Keith Joung Lab had developed SpCas9-HF1. Both of the enzymes...off-target activity relative to Cas9. Since then, the Joung and Jennifer Doudna Labs used targeted mutagenesis...also increased specificity. Most recently, the Jungjoon Lee Lab used directed evolution to generate Sniper-Cas9...29512652. PubMed Central PMCID: PMC5951633. Lee, Jungjoon K., et al. "Directed evolution of CRISPR-Cas9 ...

... Chun-Hyung Kim, Jung-Il Moon, Young-Gie Chung, Mi-Yoon Chang, Baek-Soo Han, Sanghyeok Ko, Eungi Yang,...

...and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved the on-target specificity... from the Zhang lab at the Broad Institute, the Joung lab at Harvard Medical School, and the Doudna Lab...testing of SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly thought that if they could weaken ...structural knowledge, in collaboration with the Joung Lab, they used targeted mutagenesis to create HypaCas9...