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pUb FLAG-IntS6 AA 1197-1284

Plasmid

#195074

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 1197-1284

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-IntS6 AA 101-1200

Plasmid

#195075

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 101-1200

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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hARL15_32-197

Plasmid

#209799

Purpose

Bacterial expression of human ARL15 GTPase domain

Depositor

Kalle Gehring

Article

Mahbub et al Elife. 2023 Jul 14;12:e86129. doi: 10.75

Insert

ARL15 (ARL15 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 32-197 only

Promoter

T7

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Qt-FLAG-TAA-rt20s-antiGFP

Plasmid

#206955

Purpose

Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

QtEnc, antiGFP-intrabody

Expression

Mammalian

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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PRPF4-1

Plasmid

#156158

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF4 (PRPF4 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (a)

Plasmid

#204428

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R627A K663A R683A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (b)

Plasmid

#204429

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

E626A T630A K668A E671A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-2

Plasmid

#204433

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K614A H615A D835A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Palpha6 and Interface-4 (a)

Plasmid

#204434

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

F860A Y861A Y862A Y863A Q864A R865A H866A F867A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-9

Plasmid

#204440

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

E213A Q217A R557A D565A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-CV-B3_3C

Plasmid

#203499

Purpose

Expresses CV-B3 3C protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

CV-B3 3C protease (D1P32_gp1 )

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-LV_3CL

Plasmid

#203507

Purpose

Expresses LV 3CL protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

LV 3CL protease

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-SHV_3CL

Plasmid

#203473

Purpose

Expresses SHV 3CL protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SHV 3CL protease

Expression

Yeast

Promoter

GAL10

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-HHV-3_PR

Plasmid

#203505

Purpose

Expresses protease domain of HHV-3 (VZV) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HHV-3 protease domain

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-NWV_3CL

Plasmid

#203470

Purpose

Expresses NWV 3CL protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

NWV 3CL protease

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-HTLV_PR

Plasmid

#203467

Purpose

Expresses HTLV protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HTLV protease

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-PV_3C

Plasmid

#203472

Purpose

Expresses PV 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

PV 3C protease (PVgp1 )

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-SV-A_3C

Plasmid

#203474

Purpose

Expresses SV-A 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SV-A 3C protease (Hk1_gp1 )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-1x-HIV-2_PR

Plasmid

#201935

Purpose

Expresses HIV-2 protease from a GAL-1x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HIV-2 protease

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRS193

Plasmid

#197143

Purpose

scCloneSelect C-to-T DNA barcode cloning backbone (EF1a promoter version)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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