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SFB-XPF-P379S

Plasmid

#247897

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged XPF P379S

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

XPF (ERCC4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

P379S

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SFB-XPF-D656-813

Plasmid

#247891

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged XPF D656-813

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

XPF (ERCC4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

deletion of amino acid 656-813

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SFB-XPF-R153P

Plasmid

#247893

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged XPF R153P

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

XPF (ERCC4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

R153P

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SFB-XPF-D814-916

Plasmid

#247892

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged XPF D814-916

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

XPF (ERCC4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

deletion of amino acid 814-916

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SFB-XPF-D458-655

Plasmid

#247890

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged XPF D458-655

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

XPF (ERCC4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

deletion of amino acid 458-655

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SFB-XPF

Plasmid

#247887

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged XPF

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

XPF (ERCC4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SFB-SLX4IP

Plasmid

#247870

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged SLX4IP

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

SLX4IP (C20orf94 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His-GFP-RAD54L2

Plasmid

#247924

Purpose

Insect expression construct for N-terminal His-GFP-tagged RAD54L2

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:

Insert

RAD54L2 (RAD54L2 Human)

Tags

His-GFP

Expression

Insect

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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Neo-2/15-TGFa-SPMn-SpyTag003

Plasmid

#246650

Purpose

Expresses Neo-2/15-TGFa-SPMn-SpyTag003 in mammalian cells for calcium-induced NeissLock protein ligation to epidermal growth factor receptor (EGFR)

Depositor

Mark Howarth

Article

Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021

Insert

Neo-2/15-TGFa-SPMn-SpyTag003

Tags

Signal peptide and SpyTag003

Expression

Mammalian

Mutation

Construct contains the tPA signal peptide, Neo2/…

Promoter

CMV promoter

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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tPA-TGFa-SPMn-SpyTag003

Plasmid

#246649

Purpose

Expresses tPA-TGFa-SPMn-SpyTag003 in mammalian cells for calcium-induced NeissLock protein ligation to epidermal growth factor receptor (EGFR)

Depositor

Mark Howarth

Article

Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021

Insert

tPA-TGFa-SPMn-SpyTag003

Tags

Signal peptide and SpyTag003

Expression

Mammalian

Mutation

Construct contains the tPA signal peptide, Kringl…

Promoter

CMV promoter

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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p-HITI-F9-Ckm

Plasmid

#226120

Purpose

HITI insert construct with Ckm gRNA and hF9 transgene

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Inserts

F9 (F9 Human)
Ckm gRNA (Ckm Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV_Ckm_F9

Plasmid

#226140

Purpose

AAV construct for HITI insert with Ckm gRNA and hF9 transgene

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Inserts

F9 (F9 Human)
Ckm gRNA (Ckm Mouse)
EGFP

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CMV-MCS-noATG-GFP

Plasmid

#223181

Purpose

A multicloning site (MCS) was synthesized and inserted into AAV-CMV-GFP to construct the AAV-CMV-MCS-(no ATG)-GFP negative control plasmid.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Type

Empty backbone

Use

AAV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ GFP-CDK5R1 degron (283-307)-IRES-mCherry

Plasmid

#231008

Purpose

Protein stability reporter construct for CDK5R1 consisting of aa 283-307 for transient overexpression in mammalian cells.

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

CDK5R1 aa283-307 (CDK5R1 Human)

Tags

AcGFP1

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-MCRS1

Plasmid

#231241

Purpose

Flag-tagged wild-type MCRS1 over-expression construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MCRS1 (MCRS1 Human)

Expression

Mammalian

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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AID-C12*-n'Cas9-BE4max

Plasmid

#216730

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

Depositor

Rahul Kohli

Article

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-n'Cas9-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE

Plasmid

#229544

Purpose

Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein

Depositor

Domitilla Del Vecchio

Article

Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

Insert

Oct4(co)-mNeonGreen (POU5F1 Human)

Use

Lentiviral

Tags

Oct4-mNeonGreen

Expression

Mammalian

Mutation

codon-optimized OCT4

Promoter

TRE12

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

Plasmid

#224506

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-oMDC-LexOP-c-Fos

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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TH1531-DuET

Plasmid

#213243

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

TH1531 (FZD4 Human)

Tags

1D4 and H4

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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AGTR1-DuET

Plasmid

#213183

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

AGTR1 (AGTR1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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