We narrowed to 33 results for: construct

1. Human Kinase Domain Constructs for Automated Bacterial Expression

   Plasmid Kit

   #1000000094

   Purpose

   68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expression

   Depositor

   Nicholas Levinson, John Chodera, Markus Seeliger

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

   Plasmid Kit

   #1000000171

   Purpose

   Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

   Depositor

   Johannes Stuttmann, Sylvestre Marillonnet

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

3. LbPlant toolbox II

   Plasmid Kit

   #1000000263

   Purpose

   Use the LbPlant Toolbox kits for the rapid construction of expression vectors for Lactiplantibacillus plantarum using Golden Gate cloning.

   Depositor

   Georg Fritz

   Available since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

4. MoClo Plant Parts Kit

   Plasmid Kit

   #1000000047

   Purpose

   Modular Cloning (MoClo) collection contains 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Available since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

   Plasmid Kit

   #1000000062

   Purpose

   Accessory pack for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either dCas9 or dCas9-FokI fusion; Kit #1000000055 is required to use this kit

   Depositor

   Takashi Yamamoto

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

6. EasyClone 2.0 Yeast Toolkit

   Plasmid Kit

   #1000000073

   Purpose

   Integrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strains

   Depositor

   Irina Borodina

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

7. LbPlant toolbox I

   Plasmid Kit

   #1000000262

   Purpose

   Use the LbPlant Toolbox kits for the rapid construction of expression vectors for Lactiplantibacillus plantarum using Golden Gate cloning.

   Depositor

   Thorsten Mascher

   Available since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

8. Multiplex CRISPR/Cas9 Assembly System Kit

   Plasmid Kit

   #1000000055

   Purpose

   Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack

   Depositor

   Takashi Yamamoto

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. MultiSite Gateway plasmid toolKit

   Plasmid Kit

   #1000000050

   Purpose

   Cloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modules

   Depositor

   Tomas Santalucia

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

10. CEAS (C-SWAT for Essential Allele Strains)

    Plasmid Kit

    #1000000223

    Purpose

    A suite of Saccharomyces cerevisiae strain construction vectors for perturbing gene expression at the transcriptional, post-transcriptional, and post-translational levels.

    Depositor

    Rob Mitra

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

11. Yeast GPCR-sensor Toolkit

    Plasmid Kit

    #1000000157

    Purpose

    The Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.

    Depositor

    Tom Ellis

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

12. Ralstonia-GG-Kit

    Plasmid Kit

    #1000000142

    Purpose

    Backbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniques

    Depositor

    Thomas Lahaye

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pDGE Dicot Genome Editing Kit

    Plasmid Kit

    #1000000084

    Purpose

    Golden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of “genome editing recipient” and “sgRNA shuttle” vectors.

    Depositor

    Johannes Stuttmann

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

14. FREQ-Seq Kit

    Plasmid Kit

    #1000000032

    Purpose

    For construction of barcoded, locus-specific libraries compatible with Illumina next generation sequencing. Allows for direct quantitative allele detection. 48 plasmid adaptors (barcoded M13F primer)

    Depositor

    Christopher Marx

    Available since

    May 23, 2013

    Availability

    Academic Institutions and Nonprofits only

15. MoClo Baculo toolkit

    Plasmid Kit

    #1000000256

    Purpose

    Use the MoClo Baculo Toolkit to efficiently generate multigene expression constructs for the baculovirus expression system.

    Depositor

    Chen Davidovich

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

16. Platinum Gate TALEN Kit

    Plasmid Kit

    #1000000043

    Purpose

    Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.

    Depositor

    Takashi Yamamoto

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

17. GreenGate

    Plasmid Kit

    #1000000036

    Purpose

    Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette

    Depositor

    Jan Lohmann

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

18. Golden GATEway Cloning Kit

    Plasmid Kit

    #1000000054

    Purpose

    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way

    Depositor

    Joachim Wittbrodt

    Available since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

19. UbiGate E2 activity screen (set2)

    Plasmid Kit

    #1000000145

    Purpose

    Contains bacterial constructs for E3 autoubiquitination assay, including expression cassettes containing ubiquitin and the E1 UBA1 in combination with all Arabidopsis thaliana E2s

    Depositor

    Marco Trujillo

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

20. MXS Chaining Kit

    Plasmid Kit

    #1000000064

    Purpose

    MXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signals

    Depositor

    Pierre Neveu

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only