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Plasmid#132414PurposeTo make synthetic mRNA encoding a nuclear EGFPDepositorInsertH2B-EGFP (H2BC21 Synthetic)
UseSynthetic mrna productionTagsExpressionMutationPromoterSP6Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
H2B-Grx1-roGFP2
Plasmid#236550PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleus.DepositorInsertH2B-Grx1-roGFP2 (H2BC21 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zH2B-GFP
Plasmid#226561PurposeExpress H2B-GFP in zebrafish.DepositorInserth2b (h2bc1 Zebrafish)
UseSynthetic Biology; Zebrafish expressionTagsEGFPExpressionMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA
Plasmid#225350Purposepiggyback backbone for non-fluorescent GFP tether and H2B-IRFP expressionDepositorInsertH2B (H2B Human, Synthetic)
UseTagsmiRFP and non-fluorescent GFP (Y67F)-P2AExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP H2B SL2
Plasmid#227718PurposePlasmid contains GFP H2B SL2, with some nlp-51 homology sequence after, Amp ResistantDepositorInsertGFP H2B SL2 (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
T2A GFP H2B
Plasmid#227722PurposePlasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp ResistantDepositorInsert(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)
UseCloning vectorTagsT2A 3xNLS GFPExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
SL2 GFP H2B
Plasmid#227717PurposePlasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertSL2 GFP H2B (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-H2B-MCS
Plasmid#184041PurposeCloning of GFP-H2B fusion proteinsDepositorTypeEmpty backboneUseTagsGFP and H2BExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-Aurora B KD
Plasmid#184043PurposeExpresses H2B-GFP fused to Aurora B kinase deadDepositorInsertAURKB (AURKB Human)
UseTagsEGFP and H2BExpressionMammalianMutationChanged Lysine 65 to ArgininePromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-H2B-mGFP
Plasmid#173892Purposefor H2B labelingDepositorInsertH2B (H2BC21 Human)
UseLentiviralTagsmGFPExpressionMutationPromoterSFFVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCFJ420 - Peft-3::GFP::H2B (ubiquitous)
Plasmid#34877DepositorInsertPeft-3 GFP H2B tbb-2 UTR
UseWorm targetingTagsExpressionMutationS65CPromotereef-1A.1 (eft-3)Available sinceFeb. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFJ421 - Pmyo-2::GFP::H2B (pharynx)
Plasmid#34876DepositorInsertPmyo-2 GFP H2B tbb-2 UTR
UseWorm targetingTagsExpressionMutationS65CPromotermyo-2Available sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
Gata3-T2A-H2B-eGFP donor
Plasmid#113119PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequenceDepositorInsertGata3 donor region including homology arms and T2A-H2B-eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pdgfra-T2A-H2B-eGFP donor
Plasmid#113120PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequenceDepositorInsertPdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-AsLOV2-NES27 (pDN158)
Plasmid#72659PurposeConstitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear exportDepositorInsertH2B-GFP-AsLOV2-NES27
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-EGFP
Plasmid#161776Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interestDepositorInsertH2B-EGFP from Addgene Plasmid #26790
UseLentiviral; BicistronicTagsEGFPExpressionMammalianMutationPromoterUbC and UbC promoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIN-TRE-H2BeGFP-rtTA2
Plasmid#165494PurposeDoxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.DepositorInsertHuman histone H2B (H2BC21 Human)
UseLentiviralTagsEGFPExpressionMutation-PromoterTetO-CMVAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FU-H2B-GFP-IRES-Puro
Plasmid#69550PurposeLentiviral, selectable fluorescent GFP tagging of nuclei.DepositorInsertHistone 2B - green fluorescent protein fusion
UseLentiviralTagsGFP fusionExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion T2A-H2B-GFP
Plasmid#112851PurposeGeneral donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertT2A-H2B-GFP
UseSynthetic BiologyTagsExpressionMutationPromoterNoAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B-GFP
Plasmid#116869PurposeNuclear localized GFPDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only