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Plasmid#112707PurposeExpresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cellsDepositorInsertRNMT (RNMT Human)
UseTagsFLAGExpressionMammalianMutationdeleted amino acids 2-120PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pVPSBA01-SARS-CoV2-nsp1 mutant4
Plasmid#192174Purposeexpress SARS2-NSP1-mut4DepositorInsertexpress SARS2-NSP1-mut4 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVPSBA01-SARS-CoV2-nsp1-truncated
Plasmid#192172Purposeexpress SARS2-NSP1-truncDepositorInsertexpress SARS2-NSP1-trunc (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYO1645
Plasmid#235719PurposeProtein expression of GST-tagged PX domain (Cys at 2 and C85S) in bacteriaDepositorInsertp40phox PX domain (NCF4 Human)
UseTagsGSTExpressionBacterialMutationCys at 2, C85S, aa 2-149PromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCX-OKS-2A
Plasmid#19771PurposeNon-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2DepositorUseTagsExpressionMammalianMutationThree genes are connected with the foot-and-mouth…PromoterAvailable sinceJan. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA216-CoV2-RBD-beta variant
Plasmid#194598PurposeMammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)DepositorInsertCoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-ER-mRFP
Plasmid#231180PurposeExpresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, from a lentiviral vectorDepositorInsertER-mRFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-20nm-EBFP2
Plasmid#231185PurposeExpresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nmDepositorInsert20nm-EBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-20nmEML-mRFP
Plasmid#231181PurposeExpresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 20 nm from a lentiviral vectorDepositorInsert20nmEML-mRFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ER-EBFP2
Plasmid#231183PurposeExpresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cellsDepositorInsertER-EBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-10nm-EBFP2
Plasmid#231184PurposeExpresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nmDepositorInsert10nm-EBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-3xFlag-dCas13-mTET2CD
Plasmid#228234PurposeFor targeted tethering of the catalytic domain of mouse TET2 using dCas13DepositorInsertsUseCRISPRTagsExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterCAGAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ER-mRFP
Plasmid#231176PurposeExpresses control plasmid for ER-mitochondrial linkers, tagged with mRFP, in mammalian cellsDepositorInsertER-mRFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS
Plasmid#206115Purposeexpression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surfaceDepositorInsertcacna1b (Cacna1a Rat)
UseTagsExpressionMammalianMutationE320A, E670A, E1411A, E1707APromoterCMV/B-actinAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3
Plasmid#206121Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation bindingDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationE320A, E670A, E1411A, E1707APromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-30nmEML-mRFP
Plasmid#231182PurposeExpresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 30 nm from a lentiviral vectorDepositorInsert30nmEML-mRFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-3xFlag-dCas13-mTET2HxDCD
Plasmid#228235PurposeFor targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13DepositorInsertsUseCRISPRTagsExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterCAGAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3
Plasmid#206093PurposeCav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationE314A, E664A, E1370A, E1658APromoterCMVAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS
Plasmid#206098Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surfaceDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationE314A, E664A, E1370A, E1658APromoterCMV/B-actinAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNA-NONO-1
Plasmid#127654PurposeKnock-out of human NONO (guide only)DepositorInsertNONO sgRNA (NONO Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
mini-YFP-hDF-C2F-TM
Plasmid#72600Purposemini human dysferlin (from domain F to transmembrane ) tagged with YFPDepositorInsertdysferlin (DYSF Human)
UseTagsEYFPExpressionMammalianMutationfrom domain F to transmembrane domainPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5b HA Irx5 deltaIrot
Plasmid#24995DepositorInsertIrx5 (Irx5 Mouse)
UseTagsHAExpressionMammalianMutationDeletion of Iro box (aa 314-327), 19 aa upstream …PromoterAvailable sinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXG1
Plasmid#153124PurposeExpression of Flag tagged human FOXG1 in mammalian cellsDepositorInserthuman FOXG1 (FOXG1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 Flag ATXN1[30Q]
Plasmid#33236DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsFlagExpressionMammalianMutationinsert contains a stretch of 29 amino acids (27 Q…PromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP hsp70
Plasmid#15215DepositorInserthsp70 (HSPA1A Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1-pHcRed C1
Plasmid#62959Purposefull length human Mucolipin-1 cloned into pHcRed1 C1DepositorInsertMucolipin-1 (MCOLN1 Human)
UseTagsHcRed1ExpressionMammalianMutationMucolipin-1 has 1 silent mutation compared to the…PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-hERG1a
Plasmid#53052PurposeExpresses alpha subunit of hERG1a K channel in mammalian cellsDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycIGF2BP3
Plasmid#19879DepositorInsertInsulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMXs-OLIG2
Plasmid#134668PurposeRetroviral expression of human OLIG2DepositorInsertOLIG2 (OLIG2 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-SOX10
Plasmid#134669PurposeRetroviral expression of human SOX10DepositorInsertSRY-box transcription factor 10 (SOX10 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 Flag ATXN1[2Q]
Plasmid#33235DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsFlagExpressionMammalianMutationinsert contains 2Q (2 glutamines) and there isno …PromoterCMVAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1-pEGFP C3
Plasmid#62960Purposefull length human Mucolipin-1 cloned into pEGFP C3DepositorInsertMucolipin-1 (MCOLN1 Human)
UseTagsEGFPExpressionMammalianMutationMucolipin-1 has 1 silent mutation compared to the…PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2-NOS2Promoter-Luciferase
Plasmid#19296DepositorInsertNOS2 Promoter (Nos2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HyPer-DAAO-NES
Plasmid#119164PurposeFusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.DepositorInsertHyPer-DAAO-NES
UseAAVTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Thy1 promoter construct
Plasmid#20736DepositorInsertThy1 promoter (Thy1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a
Plasmid#53051PurposeExpresses alpha subunit of hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationPromoterSP6Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SypHer2-DAAO-NES
Plasmid#119184PurposeFusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.DepositorInsertSypHer2-DAAO-NES
UseAAVTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYLL201
Plasmid#53454PurposeExpresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)InsertHA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V
Plasmid#66789PurposeExpression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoterDepositorInsertPDGFRalpha (Pdgfra Mouse)
UseTagsEGFPExpressionMammalianMutationD842V (constitutively active)PromoterEF1aAvailable sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJA219= pcDNA3.1V5-completeTRABV-CMVp
Plasmid#116878Purposemammalian expression vector containing TCR sequencesDepositorUseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe HA-Runx1-neo
Plasmid#45815DepositorInsertRunx1 (Runx1 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
145-pGL3ctrl-3' UTR
Plasmid#28175DepositorInsertLuciferase flanked by tp53 UTRs (TP53 Human)
UseLuciferaseTagsExpressionMammalianMutationNote that insert length is based on sum of the 2 …PromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycEIF2C2
Plasmid#19872DepositorInsertEukaryotic translation initiation factor 2C, 2 (AGO2 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-hDcp2 E147/8Q
Plasmid#72217Purposebacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorInsertDcp2 (DCP2 Human)
UseTagsGSTExpressionBacterialMutationE147Q, E148Q and F404L (please see depositor'…PromotertacAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
ATF4 7: 5’3’ATF4.GFP
Plasmid#21854DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationVector derived viral termination and poly-adenyla…PromoterAvailable sinceSept. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
ATF4 10: 5’ATF4.uORF1.GFP
Plasmid#21857DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationupstream ORF1 (uORF) of ATF4 fused to EGFPPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1-N1011
Plasmid#92320PurposeN-term 1-1011 of HCF-1 proteinDepositorInsertHCF-1 (HCFC1 Human)
UseTagsHAExpressionMammalianMutationAA 2-1011PromoterCMVAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
ATF4 11: 5’ATF4.uORF2.GFP
Plasmid#21858DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationupstream ORF2 (uORF) of ATF4 fused to EGFPPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprt
Plasmid#92166PurposePlasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vectorDepositorInsertQuinolinate Phosphoribosyltransferase (QPRT Human)
UseTagsTEV protease -cleavable N-terminal 6HisExpressionBacterialMutationchanged Asp 2 to Asn to create BspHI sitePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME18S-3HA-mULK2
Plasmid#22898DepositorInsertUNC-51-like kinase 2 (Ulk2 Mouse)
UseTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only