We narrowed to 28,954 results for: Met

1. TrpLE-M-nMSP1E3D1

   Plasmid

   #179949

   Purpose

   Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

   Depositor

   Kerney Glover

   Article

   Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

   Insert

   TrpLE-M-nMSP1E3D1
   

   Use

   Tags

   TrpLE

   Expression

   Bacterial

   Mutation

   Native Trp residues have been mutated to Phe resi…

   Promoter

   T7

   Available since

   Feb. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SG3ΔENV CA M66I

   Plasmid

   #149687

   Purpose

   Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

   Depositor

   Wesley Sundquist

   Article

   Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

   Insert

   Capsid
   

   Use

   Lentiviral and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed Capsid residue Met 66 to Iso (CA M66I)

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. DUSP6

   Plasmid

   #40772

   Depositor

   Dustin Maly

   Article

   Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

   Insert

   DUSP6 (DUSP6 Human)
   

   Use

   Tags

   6His and TEV

   Expression

   Bacterial

   Mutation

   Starting Met removed

   Promoter

   T7

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pGCNK58

   Plasmid

   #202461

   Purpose

   Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells

   Depositor

   Arthur Palmer, Stephen Harrison

   Article

   Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi

   Insert

   Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriEx4-H6-FGAm (FlincG3)

   Plasmid

   #49202

   Purpose

   Fluorescent reporter for cGMP

   Depositor

   John Garthwaite

   Article

   Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

   Insert

   FlincG3
   

   Use

   Tags

   His tag + other tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Met to Lys in cpEGFP region similar to CGaMP3

   Promoter

   CMV

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET51b(+)_LAMA-F98 (for GFP)

   Plasmid

   #130714

   Purpose

   Plasmid encoding the bacterial expression of LAMA-F98 for GFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   LAMA-F98
   

   Use

   Tags

   Hisx10

   Expression

   Bacterial

   Mutation

   Deleted first Met, Ala and Last Ser from the Enha…

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CMV/N3IC-HA

   Plasmid

   #47618

   Purpose

   in vitro transcription and translation of HA-tagged Notch3 Intracellular domain

   Depositor

   Urban Lendahl

   Article

   Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.

   Insert

   Notch3 Intracellular domain (Notch3 Mouse)
   

   Use

   In vitro transcription and translation

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codons 1664 (Met) to 2318 (Ala)

   Promoter

   CMV

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pET51b(+)_LAMA-G97 (for GFP)

   Plasmid

   #130715

   Purpose

   Plasmid encoding the bacterial expression of LAMA-G97 for GFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   LAMA-G97
   

   Use

   Tags

   Hisx10

   Expression

   Bacterial

   Mutation

   Deleted first Met, Ala and Last Ser from the Enha…

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET51b(+)_SNAP-p24

   Plasmid

   #130718

   Purpose

   Plasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid protein

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-p24
   

   Use

   Tags

   Hisx10 and StrepTag

   Expression

   Bacterial

   Mutation

   Deleted first Met, Ala and Last Ser from the Enha…

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET51b(+)_LAMA-N95 (for GFP)

    Plasmid

    #130716

    Purpose

    Plasmid encoding the bacterial expression of LAMA-N95 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-N95
    

    Use

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ETV3_pet28a

    Plasmid

    #131646

    Purpose

    Bacterial expression of ETS gene ETV3

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV3 (ETV3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. peT3a-AqAdk_MVGDH

    Plasmid

    #18092

    Depositor

    Dorothee Kern

    Article

    Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

    Insert

    adenylate kinase (kad A. aeolicus)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Tyr 52 changed to Cys, Val 145 changed to Cys, …

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pETM6_Ptrc_BsMdh_BmHps_BmPhi

    Plasmid

    #129104

    Purpose

    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

    Insert

    BsMDH BmHPS BmPHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDCAF3-IS5

    Plasmid

    #65220

    Purpose

    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

    Depositor

    Jeffrey Barrick

    Article

    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

    Inserts

    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed start codon from gtg to atg and Changed s…

    Promoter

    BBa_J23100

    Available since

    Jan. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pTriEx4-H6-FGAm (FlincG3)

    Plasmid

    #49202

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    FlincG3
    

    Use

    Tags

    His tag + other tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Met to Lys in cpEGFP region similar to CGaMP3

    Promoter

    CMV

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTrc99a-NoSS-TolC

    Plasmid

    #155179

    Purpose

    Expresses signal sequence-less TolC as inclusion bodies

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    TolC (tolC )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted residues 1-20, residue 21 mutated to Met …

    Promoter

    trc

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET51b(+)_LAMA-F98 (for GFP)

    Plasmid

    #130714

    Purpose

    Plasmid encoding the bacterial expression of LAMA-F98 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-F98
    

    Use

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMV/N3IC-HA

    Plasmid

    #47618

    Purpose

    in vitro transcription and translation of HA-tagged Notch3 Intracellular domain

    Depositor

    Urban Lendahl

    Article

    Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.

    Insert

    Notch3 Intracellular domain (Notch3 Mouse)
    

    Use

    In vitro transcription and translation

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codons 1664 (Met) to 2318 (Ala)

    Promoter

    CMV

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pET51b(+)_LAMA-G97 (for GFP)

    Plasmid

    #130715

    Purpose

    Plasmid encoding the bacterial expression of LAMA-G97 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-G97
    

    Use

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET51b(+)_SNAP-p24

    Plasmid

    #130718

    Purpose

    Plasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid protein

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-p24
    

    Use

    Tags

    Hisx10 and StrepTag

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge