We narrowed to 1,029 results for: gibson assembly

1. pmScarlet3-H_I3

   Plasmid

   #239343

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.

   Depositor

   Torsten Wittmann

   Insert

   I3-01
   

   Use

   Tags

   mScarlet3-H (a.k.a. mYongHong)

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmScarlet-I_I3

   Plasmid

   #239341

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet-I in mammalian cells. Assembles into nanocages tagged with 60 FPs.

   Depositor

   Torsten Wittmann

   Insert

   I3-01
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmStayGold(E138D)_I3

   Plasmid

   #239338

   Purpose

   Expresses the I3-01 nanocage subunit N-terminally tagged with mStayGold(E138D) in mammalian cells. Assembles into nanocages tagged with 60 FPs.

   Depositor

   Torsten Wittmann

   Insert

   I3-01
   

   Use

   Tags

   mStayGold(E138D)

   Expression

   Mammalian

   Mutation

   K129A

   Promoter

   CMV

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV_3XHA-ATP6V1B2

   Plasmid

   #228864

   Purpose

   Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V1B2 (ATP6V1B2 Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_3xFLAG-ATP6V0A3

   Plasmid

   #228866

   Purpose

   Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V0A3 (TCIRG1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEVOL-NBK-1

   Plasmid

   #207639

   Purpose

   A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The evolved synthetase NBK-1 contains mutations Y…

   Promoter

   araBAD

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMMK ENTR 2

   Plasmid

   #206261

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Type

   Empty backbone

   Use

   Multimate/gateway entr 2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMMK ENTR 1

   Plasmid

   #206260

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Type

   Empty backbone

   Use

   Multimate/gateway entr 1

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMMK ENTR 3

   Plasmid

   #206262

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Type

   Empty backbone

   Use

   Multimate/gateway entr 3

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMMK ENTR 4

    Plasmid

    #206263

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Multimate/gateway entr 4

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRE-mClover3

    Plasmid

    #214922

    Purpose

    expression vector control - contitutive expression of mClover3 alone

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    mClover3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TRE-mRuby3

    Plasmid

    #214923

    Purpose

    expression vector control - contitutive expression of mRuby33 alone

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    mRuby3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. R26TV LSL-TRL

    Plasmid

    #68476

    Purpose

    Rosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassette

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    tTR-KRAB-2A-rtTA3-2A-Luciferase
    

    Use

    Cre/Lox, Luciferase, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. TOPO UBC

    Plasmid

    #68456

    Purpose

    TOPO construct expressing UBC for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Ubiquitin C Promoter (UBC Human)
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag

    Plasmid

    #185683

    Purpose

    Low-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Human, Synthetic)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    (WLLI-GSGS)[359-362]

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

    Plasmid

    #193915

    Purpose

    Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO - RBM28 E5 WT minigene

    Plasmid

    #169269

    Purpose

    Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 E5 WT minigene (RBM28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 6xHis-TEV-GEI-17(133-509)

    Plasmid

    #87056

    Purpose

    Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)
    

    Use

    Tags

    6xHis-TEV and GAGGGS linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pnCS_mEGFP-DSep2_Peanut-Strep

    Plasmid

    #174496

    Purpose

    bacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila Peanut

    Depositor

    Manos Mavrakis

    Article

    Mavrakis et al Methods Cell Biol. 2016;136:199-220. doi

    Inserts

    Sep2 (Sep2 Fly)
    peanut (pnut Fly)
    

    Use

    Tags

    Strep and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. deltaVP1 + VP2C-MIOX

    Plasmid

    #166680

    Purpose

    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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