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pET28-6His-SUMO-Nsp10
Plasmid#154959PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
UseTags6His-Thrombin-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-mOct4 Full length(FL)
Plasmid#206394PurposeExpresses Oct4 with a 6xHis tag for protein purificationDepositorInsertOct4 (Pou5f1 Mouse)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-2xMreBN-mCherry-His
Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP7 (catalytic domain)
Plasmid#16360DepositorInserthuman SENP7 catalytic domain (SENP7 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 640 to 984 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL: nsp10_SARS-CoV-2
Plasmid#167851PurposeBacterial expression of the SARS-CoV-2 protein nsp10DepositorInsertnsp10
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-MHL: nsp16_SARS-CoV-2
Plasmid#167850PurposeBacterial expression of the SARS-CoV-2 protein nsp16DepositorInsertnsp16
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-6XHis-SUMO-SIRT2
Plasmid#102622PurposeExpresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purificationDepositorInsertSIRT2 (SIRT2 Human)
UseTags6XHis-SUMOExpressionBacterialMutationDeleted amino acids 1-37 and 357-389PromoterAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP2 (catalytic domain)
Plasmid#16357DepositorInserthuman SENP2 catalytic domain (SENP2 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 365 to 590 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28-T7-NlaCas3-NLS-6xHis
Plasmid#180214PurposeExpressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.ColiDepositorInsertNlaCas3c
UseTags6xHis and NLSExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His-TEV-NQO1
Plasmid#119058PurposeFor expression in E.coli and affinity purification of N-terminally His tagged NQO1DepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
UseTags6His, TEV siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-Caspase-4_enzymatic-domain
Plasmid#183381PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-4 (AA94-377) (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP1 (catalytic domain)
Plasmid#16356DepositorInserthuman SENP1 catalytic domain (SENP1 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 419 to 644 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
Pet28a-His-tag-BS2
Plasmid#220347PurposeE. coli expression vector to purify BS2DepositorInsertBS2
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceJune 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28C-SARS-CoV-2-Nucleocapsid
Plasmid#204402PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsidDepositorInsertSARS-CoV-2 nucleocapsid (N SARS-CoV-2)
UseTags6x-HisExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogTag-MBP
Plasmid#171773PurposeExpresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogTag-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-11_enzymatic-domain
Plasmid#183382PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-11 (AA100-373) (Casp4 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-TEV-Nsp14
Plasmid#154954PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsert6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
UseTags6His-TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-His6-ODC-Ctag
Plasmid#163614PurposeExpresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.DepositorInsertODC (ODC1 Human)
UseTagsC-tag and His6ExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT-L713F
Plasmid#173944PurposeBacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutationDepositorInsertPARP1-CAT-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationL713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only
