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Showing: 781 - 800 of 1402 results

  1. pAL1

    Plasmid
    #197285
    Purpose
    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195
    Depositor
    Bogumil Karas
    Article
    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.
    Insert
    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197285-plasmid-map-sequence-id-389699

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  3. pRJPaph-gusA

    Plasmid
    #67036
    Purpose
    Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Article
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Inserts
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    gusA
    Use
    Conjugative bacterial vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67036-plasmid-map-sequence-id-115551

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  4. pASKt15C+SrtA5

    Plasmid
    #64985
    Purpose
    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, D160N, D165A, K19…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64985-plasmid-map-sequence-id-109954

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  6. pDN-D2irTN2AG5kwh

    Plasmid
    #128253
    Purpose
    Mammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.
    Depositor
    Gabor Balazsi
    Article
    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:
    Insert
    Beta Globin Intron::hTetR::P2A::EGFP
    Use
    Synthetic Biology; Flp-in expression vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV D2ir promoter
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    128253-plasmid-map-sequence-id-248939

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  7. pRJPaph-mTq2

    Plasmid
    #67031
    Purpose
    Plasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Article
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Inserts
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    mTurqoise2
    Use
    Conjugative bacterial vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67031-plasmid-map-sequence-id-115541

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  8. pASKt15C+SrtA7woH

    Plasmid
    #65020
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    65020-plasmid-map-sequence-id-109964

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  10. pCS5

    Plasmid
    #55750
    Purpose
    Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.
    Depositor
    Matthew Bennett
    Article
    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50
    Insert
    KanR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Residues 59-264 of KanR
    Promoter
    T7-lac
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    55750-plasmid-map-sequence-id-86407

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  11. pTC-CMV-Tet

    Plasmid
    #85577
    Purpose
    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (CMV promoter) in mouse liver
    Depositor
    Ursula Ehmer
    Article
    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg
    Insert
    CreER
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    ApoE.HCR.hAAT
    Available since
    Jan. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85577-plasmid-map-sequence-id-169840

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  16. pRJPaph-HcRed

    Plasmid
    #67035
    Purpose
    Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Article
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Inserts
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    HcRed
    Use
    Conjugative bacterial vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    67035-plasmid-map-sequence-id-115548

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  19. pMTG8-Amp-FRT

    Plasmid
    #128277
    Purpose
    TIP-LITer2.0-KRAS(G12V) gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-KRAS(G12V)-P2AGFP
    Depositor
    Gabor Balazsi
    Article
    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527
    Inserts
    TetR-LOV-TIP
    EGFP
    KRAS(G12V)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128277-plasmid-map-sequence-id-251922

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Showing: 781 - 800 of 1402 results