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pRLCon850-1207

Plasmid

#31447

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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PGL3-Ogt (-178 to +233 bp)

Plasmid

#79266

Purpose

To identify the Cis and Trans acting elements that control Ogt gene transcription.

Depositor

Steven Jones

Article

Muthusamy et al J Biol Chem. 2015 Dec 25;290(52):31013-2

Insert

Ogt promoter element (Ogt Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only
pCRII-Plxnb3-ISH-2kb

Plasmid

#68037

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B3 (Plxnb3 Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(925-934)-NLS-mut

Plasmid

#42020

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa925-934, R930A, R931A, R932A, R934A

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4g-ISH-2kb

Plasmid

#68034

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4G (Sema4g Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pRLCon1-449

Plasmid

#31452

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-del.GW-I+II+III

Plasmid

#42033

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

deleted aa457-532, aa716-772 and aa804-924

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pRLCon1-249

Plasmid

#31453

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRLCon1-249_SNP

Plasmid

#31454

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

SNP rs11574744 in miR-34a binding site

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pFUW-SNAP25B-WT

Plasmid

#248121

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptosomal-Associated Protein (SNAP-25)

Depositor

Ege Kavalali

Article

Alten et al Neuron. 2021 Jan 6;109(1):59-72.e5. doi:

Insert

SNAP25B (SNAP25 Human)

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C82A)-flag-tev-his

Plasmid

#227166

Purpose

For overexpression of CYP2A6 C82A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 82 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C439A)-flag-tev-his

Plasmid

#227167

Purpose

For overexpression of CYP2A6 C439A mutant in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Mutation

changed cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-cyp-33e1-flag-tev-halo-his

Plasmid

#227151

Purpose

For T-REX experiments of cyp-33e1. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-aex-5-flag-tev-halo-his

Plasmid

#227152

Purpose

For T-REX experiments of aex-5.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

aex-5 (aex-5 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6-flag-tev-halo-his

Plasmid

#227154

Purpose

For T-REX experiments of CYP2A6. A reactive electrophilic species (RES) Sensor.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-21222-SIN3A-3xFLAG

Plasmid

#109213

Purpose

Encodes human full-length SIN3A with a C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length SIN3A (SIN3A Human)

Tags

3xFLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-21222-SIN3B-3xFLAG

Plasmid

#109214

Purpose

Encodes human full-length SIN3B with a C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Jayaprakash et al Mol Biotechnol. 2021 Jan;63(1):53-62. do

Insert

human full-length SIN3B (SIN3B Human)

Tags

3xFLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMTOR_1k)-PGKpuro2ABFP-W

Plasmid

#208429

Purpose

Lentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMTOR_2b)-PGKpuro2ABFP-W

Plasmid

#208430

Purpose

Lentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCHC022 (∆phcA)

Plasmid

#226200

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for PhcA

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC004 (∆phaCAB)

Plasmid

#226198

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for phaCAB

Use

Synthetic Biology

Available Since

Nov. 15, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMXS-IRES-Blast coUCK2 D62A

Plasmid

#211530

Purpose

Expresses codon optimized catalytic mutant UCK2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

UCK2 D62A (UCK2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

D62A (catalytic mutant)

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_E192K_D193K

Plasmid

#214322

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

E192K_D193K

Promoter

T7

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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rSirt3 shRNA (pLKO.1 vector)

Plasmid

#216807

Purpose

shRNA against rat Sirt3

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

Insert

Sirt3 (Sirt3 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL-18_I48N

Plasmid

#214323

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

I48N

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_mouse-Pro-IL-18_humanized-triple-mutant

Plasmid

#214339

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (Il18 Mouse)

Tags

His-TEV

Expression

Bacterial

Mutation

ENGD29DDEN_N36Y_H41E

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_human-pro-IL-18_LES33AAA

Plasmid

#214326

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

LES33AAA

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_human-pro-IL-18_DDEN29ENGD

Plasmid

#214325

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

DDEN29ENGD

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_human-pro-IL-18_V47N_I48N

Plasmid

#214324

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

V47N_I48N

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_rat

Plasmid

#214333

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (Il18 Rat)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge