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pTH399-SUP45-S30P

Plasmid

#29372

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

S30P mutant gene (corresponding to the sl23ts all…

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pTH375-SUP45-P174Q

Plasmid

#29383

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Expression

Yeast

Mutation

P174Q mutant gene including genomic sequence 1432…

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pUC_delta ald1

Plasmid

#126708

Purpose

vector for knocking out ald1 in Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

HIS4

Expression

Yeast

Promoter

His

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-GST

Plasmid

#39286

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8-MET15

Plasmid

#207032

Purpose

Expression of 3HA-Atg8.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Promoter

pATG8

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pWR23

Plasmid

#202770

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coKanMX
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-PGAL-GFP-Cps1

Plasmid

#207030

Purpose

Expression of GFP-Cps1 under GAL promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Cps1

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCTCON2-Aga2p-Flag-CapN-TEVcs-HA

Plasmid

#213530

Purpose

Expresses CapN-caged TEVcs on yeast surface

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

Aga2p-Flag-CapN-TEVcs-HA

Expression

Yeast

Promoter

Gal1/10

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-4V5-Pho8-Met

Plasmid

#207029

Purpose

Expression of 4V5-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xV5

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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BS-Trp-PCUP1-4myc-Pho8

Plasmid

#207028

Purpose

Expression of 4Myc-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

4xMyc

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-Vac8-GFP-Ura

Plasmid

#207027

Purpose

Expression of Vac8-GFP under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vac8

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-Vma13-Ura

Plasmid

#207026

Purpose

Expression of GFP-Vma13 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Vma13 ORF

Tags

GFP

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-mRuby3-Pho8-406

Plasmid

#207043

Purpose

Expression of mRuby3-Pho8 under CPU1 promoter.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Tags

mRuby3

Expression

Yeast

Promoter

pCUP1

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCOA-Leu-FvPPT1

Plasmid

#179923

Purpose

Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

Depositor

Jens Laurids Sørensen

Article

Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

Insert

FvPPT1

Expression

Yeast

Mutation

Codon optimized for expression in Saccharomyces c…

Promoter

Yarrowia lipolytica EXP1

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-GGA2-GAE

Plasmid

#199175

Purpose

Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

GGA2-GAE domain (GGA2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Contains Lys 545 Gln substitution

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-GGA3-GAE

Plasmid

#199176

Purpose

Expression of GAL4 DNA-binding domain (BD)-GGA3-GAE domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

GGA3-GAE domain (GGA3 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-γ2 ear

Plasmid

#199178

Purpose

Expression of GAL4 DNA-binding domain (BD)-AP-1 γ2 ear domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

AP-1 γ2 ear domain (AP1G2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-GGA1-GAE

Plasmid

#199174

Purpose

Expression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

GGA1-GAE domain (GGA1 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ1B (137-423)

Plasmid

#198172

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1B (137-423)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-AAGAB

Plasmid

#198332

Purpose

Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AAGAB (AAGAB Human)

Tags

GAL4-DNA binding domain (BD) fragment

Expression

Yeast

Promoter

ADH1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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