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pACYC.Taq.1Amb

Plasmid

#102794

Purpose

Expresses Taq DNA polymerases with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Mutation

W168Stop; *(see below)

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-AP.Agmat.1-GCaMP7f-WPRE

Plasmid

#211492

Purpose

expression of GCaMP7f under artificial promoter Agmat

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GCaMP7f

Use

AAV

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-PLEKHG4

Plasmid

#129618

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

PLEKHG4

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSuper-gmiR-1

Plasmid

#171553

Purpose

a human miR-1-expressing plasmid

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

miR-1-2 (MIR1-2 Human)

Expression

Mammalian

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-PLEKHG2

Plasmid

#129617

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

PLEKHG2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-luc-miR-122

Plasmid

#171557

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-122

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-122

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Citrine-N1

Plasmid

#54593

Purpose

Localization: N1 Cloning Vector, Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

Citrine

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCherry-PLEKHG1

Plasmid

#129616

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

PLEKHG1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Citrine-pBAD

Plasmid

#54772

Purpose

Localization: Protein Expression Vector , Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

6xHis and Citrine

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pQE-WT-CGG-R12-KIRV

Plasmid

#63668

Purpose

CGG-R12-KIRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CGG-R12-KIRV RNAP

Mutation

Q744K, L747V, N748H, L749I, R756E, L757M, H772R, …

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pRVdG-N-P-M-EGFP-SynPhRFP-L

Plasmid

#52483

Purpose

Expresses EGFP and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-FGD5

Plasmid

#129613

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

FGD5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCM-HA-Gas1

Plasmid

#38313

Depositor

Susan Lindquist

Article

McLellan et al ACS Chem Biol. 2012 Jun 22.

Insert

Gas1 (GAS1 Budding Yeast)

Tags

2xHA

Mutation

contains promoter region

Promoter

Endogenous

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pcNB3

Plasmid

#89243

Purpose

Resurfaced Cell-penetrating B-lac nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB3 (B-Lac)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pRVdG-EGFP-N-P-M-SynPhRFP-L

Plasmid

#52479

Purpose

Expresses EGFP and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pCF711

Plasmid

#121662

Purpose

U6-sgRNA EFS-ProCas9-FlaviS6-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

U6

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-ARF1a

Plasmid

#67493

Purpose

Expression of shRNA targeting human ARF1a

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF1 shRNA

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
hs-calx1

Plasmid

#1740

Depositor

Craig Montell

Article

Wang et al Neuron 2005 Feb 3;45(3):367-78.

Insert

calx1 (Calx Fly)

Expression

Insect

Mutation

N/A

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
hs-calx2

Plasmid

#1741

Depositor

Craig Montell

Article

Wang et al Neuron 2005 Feb 3;45(3):367-78.

Insert

calx2 (Calx Fly)

Expression

Insect

Mutation

N/A

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pGL-Rbl2

Plasmid

#20885

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

Rbl2 (p130) 3'UTR (Rbl2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only