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pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

Plasmid

#181975

Purpose

Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

Depositor

Neville Sanjana

Publication

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

FMC6.3-BBz CAR; tNGFR (NGFR Synthetic, Human)

Use

Lentiviral

Mutation

NGFR truncation: aa 11-276

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTR01F/hFGFR1c-V5

Plasmid

#236011

Purpose

for PiggyBac mediated integration and stable expression of hFGFR1c protein

Depositor

Pavel Krejčí

Publication

Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

Insert

human FGFR1c (FGFR1 Human)

Tags

V5/His

Expression

Mammalian

Promoter

truncated CMV promoter

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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LLP994

Plasmid

#239916

Purpose

sgRNA-D compatible with TCTP-SynPro-01, TCTP-SynPro-16 and CaMV 35S-SynPro-11 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA-D

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP993

Plasmid

#239915

Purpose

sgRNA-C compatible with TCTP-SynPro-01, TCTP-SynPro-17 and CaMV 35S-SynPro-11 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA-C

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1007

Plasmid

#239929

Purpose

sgRNA compatible with TCTP-SynPro-04, TCTP-SynPro-11 and CaMV 35S-SynPro-08 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Publication

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA

Use

Synthetic Biology

Mutation

N/A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTR01F/hFGFR1b-V5

Plasmid

#236012

Purpose

for PiggyBac mediated integration and stable expression of hFGFR1b protein

Depositor

Pavel Krejčí

Publication

Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

Insert

human FGFR1b (FGFR1 Human)

Tags

V5/His

Expression

Mammalian

Promoter

truncated CMV promoter

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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BPK1520-sgRNA GLB1

Plasmid

#184378

Purpose

Expresses a sgRNA to edit GLB1 gene NM_000404.2_c.907A>G

Depositor

David Gilot

Publication

Leclerc et al CRISPR J. 2023 Feb;6(1):17-31. doi: 10.1

Insert

sgRNA targeting GLB1 (GLB1 Human)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pTR01F/hFGFR3b-V5

Plasmid

#236016

Purpose

for PiggyBac mediated integration and stable expression of hFGFR3b protein

Depositor

Pavel Krejčí

Publication

Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

Insert

human FGFR3b (FGFR3 Human)

Tags

V5/His

Expression

Mammalian

Promoter

truncated CMV promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DjCas13d-P2A-mCherry-pA

Plasmid

#233027

Purpose

To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Publication

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d/mCherry

Use

AAV

Tags

mCherry

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI) 0.5 Syn-intron-CasRx-pA

Plasmid

#233038

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a 0.5 bp synapsin promoter

Depositor

Jonathan Ploski

Publication

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and CasRx

Use

AAV

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-hfCas13d-P2A-mCherry-pA

Plasmid

#233026

Purpose

To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Publication

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d/mCherry

Use

AAV

Tags

mCherry

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa

Plasmid

#233032

Purpose

To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome

Depositor

Jonathan Ploski

Publication

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx and mCherry

Use

AAV

Tags

mCherry

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-7b [N194/11R]

Plasmid

#206540

Purpose

Mammalian Expression Plasmid of anti-QKI-7b (Human). Derived from hybridoma N194/11.

Depositor

James Trimmer

Publication

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-QKI-7b (Homo sapiens) recombinant Mouse monoclonal antibody (QKI Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-W160L+W275L

Plasmid

#196021

Purpose

Expresses CRKL W160L + W275L

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3_CRKL-silent-V5

Plasmid

#196015

Purpose

Expresses CRKL-silent, V5 tagged

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3_CRKL-W160L-V5

Plasmid

#196017

Purpose

Expresses CRKL W160L, V5 tagged

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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plx304_CRKL-open-V5

Plasmid

#196023

Purpose

Expresses CRKL open, V5 tagged

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Lentiviral

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-W160L+Y207F

Plasmid

#196020

Purpose

Expresses Expresses CRKL W160L + Y207F

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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W118-1_eGFP

Plasmid

#180379

Purpose

lentiviral transduction of eGFP gene

Depositor

Huda Zoghbi

Publication

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

eGFP (eGFP )

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_R49H

Plasmid

#180382

Purpose

lentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domain

Depositor

Huda Zoghbi

Publication

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_R49H (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R49H

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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