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pET15bLCFPT7

Plasmid

#53545

Purpose

Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant

Depositor

Lingchong You

Article

Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

Insert

T7 RNAP

Expression

Bacterial

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pHis-zfCSF3a

Plasmid

#65612

Purpose

express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

Insert

Csf3a (csf3a Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pHis-zfCSF3a

Plasmid

#65612

Purpose

express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

Insert

Csf3a (csf3a Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
DL147_plenti_TRE_HT-iPAK4

Plasmid

#187447

Purpose

Tet-On inducible expression of HaloTag-iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box Pak4

Use

Lentiviral

Tags

halo tag

Expression

Mammalian

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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DL147_plenti_TRE_HT-iPAK4

Plasmid

#187447

Purpose

Tet-On inducible expression of HaloTag-iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box Pak4

Use

Lentiviral

Tags

halo tag

Expression

Mammalian

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Integrin-αv Ectodomain M400C

Plasmid

#159642

Purpose

Contains integrin alpha v residues 1–960 with the M400C mutation

Depositor

Timothy Springer

Article

Wang et al Proc Natl Acad Sci U S A. 2017 May 23;11

Insert

Integrin alpha v ectodomain

Expression

Mammalian

Mutation

M400C

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Integrin-αv Ectodomain M400C

Plasmid

#159642

Purpose

Contains integrin alpha v residues 1–960 with the M400C mutation

Depositor

Timothy Springer

Article

Wang et al Proc Natl Acad Sci U S A. 2017 May 23;11

Insert

Integrin alpha v ectodomain

Expression

Mammalian

Mutation

M400C

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo6

Plasmid

#203957

Purpose

Cloning plasmid containing the kanamycin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Kanamycin Resistance Gene

Expression

Plant

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo6

Plasmid

#203957

Purpose

Cloning plasmid containing the kanamycin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Kanamycin Resistance Gene

Expression

Plant

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXY1

Plasmid

#90487

Purpose

Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pXY1

Plasmid

#90487

Purpose

Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pXY1-spc

Plasmid

#90488

Purpose

Plasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pXY1-spc

Plasmid

#90488

Purpose

Plasmid with ptb promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Type

Empty backbone

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GdT

Plasmid

#87361

Purpose

Expresses GdT, a dimeric green fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GdT

Tags

His-Tag

Expression

Bacterial

Promoter

AraBad

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GdT

Plasmid

#87361

Purpose

Expresses GdT, a dimeric green fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GdT

Tags

His-Tag

Expression

Bacterial

Promoter

AraBad

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCas9–mKate2ps–CgRNA

Plasmid

#64955

Purpose

Control gRNA (GTCAAGGCACTCTTGCCTA)

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCas9–mKate2ps–CgRNA

Plasmid

#64955

Purpose

Control gRNA (GTCAAGGCACTCTTGCCTA)

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pdestMB14

Plasmid

#26415

Depositor

Marc Vidal

Article

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2

Expression

Bacterial and Worm

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pdestMB14

Plasmid

#26415

Depositor

Marc Vidal

Article

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2

Expression

Bacterial and Worm

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pSY45

Plasmid

#48355

Purpose

Targets TbMCM-BP for Cre-lox mediated knockout in T. brucei

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

loxP-SAS-HYG-Ty1-TK-loxP flanked by TbMCM-BP-targeting homologies

Use

Cre/Lox; Knockout in t. brucei

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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