We narrowed to 14,321 results for: cas9

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tRNA-gRNA position [n] (GB1207)

Plasmid

#75408

Purpose

tRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA position [n]

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pV1025

Plasmid

#111424

Purpose

Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)

Depositor

Gerald Fink

Publication

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

CaCas9

Use

CRISPR

Expression

Yeast

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ256

Plasmid

#124312

Purpose

Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing

Depositor

Yiping Qi

Publication

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A-PmCDA1-UGI

Use

CRISPR

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97311

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97312

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HMEJ donor

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ai14-HITI

Plasmid

#87117

Purpose

AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-Ai14sgRNA-GFPNLS-EF1a-mCherryKASH-pA

Use

AAV and CRISPR

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ230-TREX2-N

Plasmid

#244025

Purpose

Gateway entry plasmid (attL1& attR5) expressing TREX2 exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Publication

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas12a

Use

CRISPR

Tags

TREX2

Available since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ230-AtEXO1B-N

Plasmid

#244024

Purpose

Gateway entry plasmid (attL1& attR5) expressing AtEXO1B exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Publication

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas12a

Use

CRISPR

Tags

AtEXO1B

Available since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB U6

Plasmid

#229956

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Publication

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB EF1alpha

Plasmid

#229954

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Publication

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE

Plasmid

#229955

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning

Depositor

Martin Pelosse

Publication

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pWR95(URA3)

Plasmid

#203917

Purpose

BLINCAR plasmid for gene deletion, URA3 target

Depositor

J. Brian Robertson

Publication

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

URA3 upstream homology
URA3 downstream homology

Expression

Yeast

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR23

Plasmid

#202770

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Publication

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coKanMX
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR10

Plasmid

#202710

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Publication

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coShBle
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR9

Plasmid

#202645

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Publication

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR54

Plasmid

#203330

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Publication

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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EC67900

Plasmid

#209494

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

HvU3

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC70210

Plasmid

#209478

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70123

Plasmid

#209481

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70124

Plasmid

#209482

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU3

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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