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SITS-ccdb-mCherry

Plasmid

#118753

Purpose

Multisite gateway destination vector for 3' tagging with mCherry for cell free expression. Parton lab clone FFF

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

mCherry

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S10

Plasmid

#128594

Purpose

Expresses tagless S10 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S10 (rpsJ E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S3-M129C

Plasmid

#132949

Purpose

Expresses tagless S3-M129C mutant ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

E.coli ribosomal protein S3 (rpsC E.coli)

Expression

Bacterial

Mutation

M129C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S3

Plasmid

#128591

Purpose

Expresses tagless S3 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S3 E.coli ribosomal protein (rpsC E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-MiniE-mEm

Plasmid

#181991

Purpose

MiniE genetically encoded tag on H2B-mEmerald to be used with MiniR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

H2B-MiniE-mEm

Tags

mEmerald

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat_57-499_B

Plasmid

#145978

Purpose

Insect Expression of DmHPat_57-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-499 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat_1-56_A

Plasmid

#145869

Purpose

Insect Expression of DmHPat_1-56

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_1-56 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat_1-499_A

Plasmid

#145871

Purpose

Insect Expression of DmHPat_1-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_1-499 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat_226-499_A

Plasmid

#145872

Purpose

Insect Expression of DmHPat_226-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_226-499 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat_226-499_A

Plasmid

#145877

Purpose

Insect Expression of DmHPat_226-499

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_226-499 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat_500-968_A

Plasmid

#145878

Purpose

Insect Expression of DmHPat_500-968

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_500-968 (Patr-1 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-td-sfGFP

Plasmid

#180150

Purpose

td-sfGFP cre reporter

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

td-sfGFP

Use

Cre/Lox

Expression

Mammalian

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-CAAAG

Plasmid

#170096

Purpose

deGFP reporter plasmid with CAAAG PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-CAATG

Plasmid

#170097

Purpose

deGFP reporter plasmid with CAATG PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-GTAAT

Plasmid

#170098

Purpose

deGFP reporter plasmid with GTAAT PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-GTATT

Plasmid

#170099

Purpose

deGFP reporter plasmid with GTATT PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA2

Plasmid

#170089

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-ATAAC

Plasmid

#170095

Purpose

deGFP reporter plasmid with ATAAC PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEPV-FLAG-DsRed2-ER-5

Plasmid

#170621

Purpose

Expresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

EPV-FLAG-DsRed2-ER-5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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PROM5_MprbcL_short_chlo

Plasmid

#165161

Purpose

L0 part - promoter

Depositor

Jim Haseloff

Article

Frangedakis et al ACS Synth Biol. 2021 Jul 16;10(7):1651-1

Insert

Marchantia polymorpha rbcL promoter region

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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