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huCof C39A,C147A-mRFP

Plasmid

#245972

Purpose

Expression of cof with two of 4 cys mutated to A. Does NOT incorporate into cofilin-actin rods .

Depositor

James Bamburg

Article

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C39A,C147A

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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huCof C39A-mRFP

Plasmid

#245966

Purpose

Expression of cof with one of 4 cys mutated to ala. Incorporates into cofilactin rods

Depositor

James Bamburg

Article

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C39A

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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huCof C39S-mRFP

Plasmid

#245967

Purpose

Expression of cof with one of 4 cys mutated to ser. Incorporates into cofilactin rods

Depositor

James Bamburg

Article

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C39S

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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huCof C139A-mRFP

Plasmid

#245968

Purpose

Expression of cof with one of 4 cys mutated to ala. Incorporates into cofilactin rods

Depositor

James Bamburg

Article

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C139A

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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huCof K95Q-mRFP

Plasmid

#245974

Purpose

Expression of cofilin with mutation in one actin binding domain

Depositor

James Bamburg

Article

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

K95Q

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pSG5-CAGEc-PRKACA (Nrdj1)

Plasmid

#241421

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product DNAJ-PKAc, Figure 4, Extended Figure 8-9 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

CAGEc-PRKACA (Nrdj1) (PRKACA Human, Synthetic)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244083

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(mem).iGlucoSnFR2.mIRFP670nano3

Plasmid

#244102

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mIRFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244089

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244090

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Tags

IgG kappa leader and mIRFP670nano3

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NES-CAGEc-HOXA9c (Nrdj1)

Plasmid

#241416

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Figure 2 & Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

NES-CAGEc-HOXA9c (Nrdj1) (HOXA9 Human, Synthetic)

Tags

3xFLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NES-CAGEc-HOXA9c (Npu)

Plasmid

#241415

Purpose

Proximity-triggered Protein Trans-Splicing to generate the splice product MLL-AF9, Extended Figure 4 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

NES-CAGEc-HOXA9c (Npu) (HOXA9 Human, Synthetic)

Tags

3xFLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pVBL0001

Plasmid

#242429

Purpose

Stable genomic integration of Opto-IRE1 (HsIRE1dLD-mCherry-CRY2clust) using the Flp-in system.

Depositor

Vladislav Belyy

Article

Smith et al bioRxiv [Preprint]. 2025 Jul 21:2025.07.

Insert

IRE1alpha (ERN1 Human)

Tags

mCherry, CRY2clust

Expression

Mammalian

Mutation

Deletion of the lumenal domain

Promoter

CMV, tet-inducible

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_delta_EHMT1

Plasmid

#228816

Purpose

Expression of human EHMT1 with deletion of region between N-terminal 300 aa and C-terminal Ank+SET

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of region between N-terminal 300 aa and …

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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TTR F87E

Plasmid

#238075

Purpose

Bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

F87E

Promoter

T7

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG2(WT)

Plasmid

#79432

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG2(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG2 (ARFGEF2 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG1(WT)

Plasmid

#79431

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG1 (ARFGEF1 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Mutation

C1758R

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 FLAG CEP68

Plasmid

#236463

Purpose

transient overexpression of Cep68 in mammalian cells

Depositor

Michele Pagano

Article

Pagan et al Nat Cell Biol. 2015 Jan;17(1):31-43. doi

Insert

Cep68 (CEP68 Human)

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGM63

Plasmid

#220169

Purpose

CRISRPi guide RNA against TOMM70A

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM70A (sgRNA) (TOMM70 Human)

Use

Lentiviral

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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MFE23-ZZ

Plasmid

#223553

Purpose

MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.

Depositor

Jean Gariepy

Article

Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

Insert

pET302-MFE23-ZZ

Tags

HA tag and HIS tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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