We narrowed to 14,430 results for: nts

1. pYPQ262m

   Plasmid

   #161522

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE7.10 mediated A-G base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   TadA(wt)-TadA(7.10)-zSpCas9(D10A)
   

   Use

   CRISPR; Gateway compatible tada(wt)-tada(7.10)-zs…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Venus-Bik-pEGFP-C1

   Plasmid

   #166737

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Bik (BIK Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-G2ZYP2

   Plasmid

   #103110

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE Tight ArchT-GFP

   Plasmid

   #79627

   Purpose

   Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

   Depositor

   Yasunori Hayashi

   Article

   Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

   Insert

   ArchT
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   ArchT is a more sensitive version of Arch (archae…

   Promoter

   Tet responsive Ptight promoter

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

5. Cry2 mCherry PGK-1 in pcDNA3.1

   Plasmid

   #64214

   Purpose

   mCherry fused between Cry2 and PGK-1 to study cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

   Inserts

   Cry2 PHR (CRY2 Mustard Weed)
   PGK1 (PGK1 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCEP4-myc-HLA-A2-VN

   Plasmid

   #135485

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-mqgDUX4

   Plasmid

   #21176

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

   Insert

   DUX4 (DUX4 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pUC19-Q927P4

   Plasmid

   #103131

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. (517) SB KRAB-SadCas9 U6-gStop

   Plasmid

   #163023

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3_AVPR2-NTEV-TCS-GV-2xHA

    Plasmid

    #194370

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    AVPR2-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. FB007

    Plasmid

    #119705

    Purpose

    Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Promoter gpdA
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    gpdA

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126778

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R691A

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ZIKV_NASBA_32B

    Plasmid

    #75011

    Purpose

    Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_NASBA_32B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221 NLRP1 P1214R

    Plasmid

    #166822

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation P1214R (Grandemange et al., 2017) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    P1214R

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiU6-mNeurog2 gRNA_1-MS2-Puro

    Plasmid

    #192680

    Purpose

    Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. KHBD00482

    Plasmid

    #39590

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG12287 (pdm2 Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

17. ZIKV_NASBA_27B

    Plasmid

    #75010

    Purpose

    Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_NASBA_27B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. KHBD00642

    Plasmid

    #39706

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5748 (Hsf Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pSC4

    Plasmid

    #91182

    Purpose

    T-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNAs targeting 58kb region in medicago truncatula
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCK669

    Plasmid

    #192638

    Purpose

    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dxCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    SoxS has R93A and S101A mutations and dxCas9-NG h…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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