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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

Plasmid

#129039

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA11 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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peDK.11

Plasmid

#112150

Purpose

"Vector DK"

Depositor

Stephen Harrison

Article

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Type

Empty backbone

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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MYMH 11

Plasmid

#138020

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-2AG-GFP

Expression

Bacterial

Mutation

AGA GAG GAG AAA

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

Plasmid

#139096

Purpose

Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

KRAB-dxCas9 3.7

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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C-11-BE

Plasmid

#135039

Purpose

C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9

Depositor

Tian-Lin Cheng, Zilong Qiu

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L2_2

Use

CRISPR

Expression

Mammalian

Promoter

U6 with EF-1alpha intron

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.11

Plasmid

#133532

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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SG-C-Int-11

Plasmid

#117040

Purpose

Constitutive integrase cassette - Bxb1-Tp901-Int5

Depositor

Jerome Bonnet

Article

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Bxb1-Tp901-Int5

Use

Synthetic Biology

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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PE_11: Para_lac_tet_bD

Plasmid

#113596

Purpose

PE_11: Para_lac_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Para_lac_tet_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Para_lac_tet_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pUPD-pGAL1 (01-11)

Plasmid

#101682

Purpose

pGAL1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YBR020W (455bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pTDH3 (01-11)

Plasmid

#101683

Purpose

pTDH3 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YGR192C (675bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pTDH2 (01-11)

Plasmid

#101684

Purpose

pTDH2 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YJR009C (699bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pHXT7 (01-11)

Plasmid

#101686

Purpose

pHXT7 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YDR342C (587bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pTPI1 (01-11)

Plasmid

#101687

Purpose

pTPI1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YDR050C (918bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-pPYK1 (01-11)

Plasmid

#101688

Purpose

pPYK1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YAL038W (727bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD-PGI1 (01-11)

Plasmid

#101690

Purpose

pPGI1 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YBR196C (402bp)]-CCAT

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRS316Nab3-11

Plasmid

#110535

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Mutation

two missense mutations creating Nab3-11

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3-11

Plasmid

#110534

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Mutation

two missense mutations creating Nab3-11

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pFusB3-11

Plasmid

#63496

Purpose

Encodes RVD positions 13-15 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NI NN NN

Use

TALEN

Mutation

none

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pFusB2-11

Plasmid

#63480

Purpose

Encodes RVD positions 13-14 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NN NN

Use

TALEN

Mutation

none

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pFusX4-11

Plasmid

#63420

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

NI NN NN

Use

TALEN

Mutation

none

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only