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Plasmid#68698PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only
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pMpGWB410
Plasmid#68675PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB413
Plasmid#68678PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB233
Plasmid#68624PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB210
Plasmid#68601PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TRP32
Plasmid#71730Purposeinducible expression of TRP32 in E.coliDepositorInsertTRP32
UseTags6xHIS and ThioredoxinExpressionBacterialMutationPromoterpBADAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SNV-memTdTomato-2AcytoGFP
Plasmid#53124PurposeRetrovirus vector backbone that co-expresses GFP and a gene of interest fused to memTdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorTypeEmpty backboneUseRetroviral; Expression in chicken cellsTags2A-cyto-GFP and memTdTomatoExpressionMutationPromoterSNV (spleen necrosis virus)Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNV-memTdTomato
Plasmid#53123Purposeretroviral vector expressing membrane TdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cellsDepositorInsertmemTdTomato
UseRetroviral; Expression in chicken cellsTagsExpressionMutationPromoterSNV (spleen necrosis virus)Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SpEAF pBacPAK8
Plasmid#15319DepositorInsertELL associated factor (eaf1 Fission Yeast)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseTagsExpressionBacterialMutationPromoternative promoterAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby
Plasmid#71760PurposemGFP and Synaptophysin-mRuby expression from Cre-expressing neuronsDepositorHas ServiceAAV1InsertsmGFP
Synaptophysin
UseAAV and Cre/LoxTagsPalmitoylation signal and mRubyExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 F-tractin-EGFP
Plasmid#58473PurposeExpresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoterDepositorInsertF-tractin (Itpka Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB533-44B12m23-sfGFP
Plasmid#186778PurposeRNAP2 Ser5ph-mintbody expression vector for mammalian cellsDepositorInsert44B12m23-scFv
UseTagssfGFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-mCherry
Plasmid#193293Purposeepisomal vector expressing mCherry fluorescent proteinDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_His-TEV-EGFP
Plasmid#223723PurposeExpression of recombinant protein for purificationDepositorInsertEGFP, not monomeric (has A207)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
SuperNova/pRSETB
Plasmid#53234Purposemonomeric photosensitizing fluorescent protein for chromophore-assisted light inactivationDepositorInsertmonomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVH64-Tier1-PSV40-Citrine_p2a_SEAP
Plasmid#169558PurposeTier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).DepositorInsertPSV40-driven citrine-P2A-SEAP fusion construct
UseTagsExpressionMammalianMutationPromoterPSV40Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB120
Plasmid#68574PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Green Pegassos
Plasmid#163114PurposeExpresses biosensors for pyruvate in mammalian cellsDepositorInsertGreen Pegassos
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-GFP
Plasmid#39289DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locusPromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-EGxxFP-iP-A
Plasmid#204624PurposeCloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV-PGKpuro2ABFP
Plasmid#122372PurposeExpresses puro-2A-BFP in mammalian cellsDepositorInsertPuro-2A-BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Green Lindoblum
Plasmid#163113PurposeExpresses biosensor for lactate in mammalian cellsDepositorInsertGreen Lindolum
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEC3079 (p7INT-Pveg_lacI)
Plasmid#218505Purposeintegrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorInsertlacI (lacI Escherichia coli)
UseTagsExpressionBacterialMutationPromoterPvegAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRII_TOPO_hNEAT1
Plasmid#61518Purposehuman NEAT1_v1 short isoform 3.7kb insert in general cloning vectorDepositorInsertNEAT1 (NEAT1 Human)
UseGeneral cloning vectorTagsExpressionMutation*see comment belowPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBa.PEX3-tdTomato-FKBP
Plasmid#193941PurposeMammalian expression of tdTomato-tagged PEX3DepositorInsertPEX3 (PEX3 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti EF1a GFP-mCherry-mPlin1
Plasmid#189005PurposeFluorescent reportor for lipophagyDepositorInsertGFP, mChery, Plin1 (Plin1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-2-EWSR1
Plasmid#46384DepositorInsertEWSR1 (EWSR1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL
Plasmid#169533PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).DepositorInsertPCMV-driven ER-localized monomeric red fluorescent protein mRuby2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDL17
Plasmid#106483PurposepDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosomeDepositorInsertsgam
bet
exo
lacI
rhaR
rhaS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-dPspCas13b-NES-mCherry
Plasmid#233609PurposeExpression of catalytically-inactive PspCas13b in mammalian cellsDepositorInsertdPspCas13b-NES-mCherry
UseCRISPRTags3xFlag and mCherryExpressionMutationH133A/H1058APromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-dPspCas13b-4EHP-NES-mCherry
Plasmid#233610PurposeExpression of catalytically-inactive PspCas13b fused with 4EHP in mammalian cellsDepositorInsertdPspCas13b-4EHP-NES-mCherry
UseCRISPRTags3xFlag and mCherryExpressionMutationH133A/H1058APromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010
Plasmid#71536PurposeFor CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBRα-σ70 D581G
Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
UseTagsExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-SHH
Plasmid#134350PurposeTargeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locusDepositorInsertSHH (SHH Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1
Plasmid#39280DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locusPromoterAvailable sinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pME-nls-sfGFP
Plasmid#132973Purposefull-length of nuclear localized sfGFP with stop codon in pmE Gateway vectorDepositorInsertnuclear localized super folder GFP
UseGateway vectorTags6x myc and SV40 NLSExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p15aC-4D1D-5
Plasmid#107866PurposeBacterial expression of PIS, PI4K and PI4P5KDepositorInsertsPIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 4-kinase β
UseTagsmycExpressionBacterialMutationPromoterproD and proD (in operon)Available sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB115
Plasmid#68569PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pGAP
Plasmid#98498PurposeBB1 with insert: promoter PGAPDepositorInsertGAP promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB116
Plasmid#68570PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry2-mito
Plasmid#164164PurposeExpresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoterDepositorInsertmCherry2-mtActA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGE011
Plasmid#71537PurposeFor CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB215
Plasmid#68606PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Promoter-4xBS_BHLHE40
Plasmid#153064PurposeFour BHLHE40 consensus binding sites cloned upstream of luciferase reporter geneDepositorInsert4X consensus BHLHE40 binding sites
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Utr230-EGFP
Plasmid#58472PurposeExpresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoterDepositorInsertUtr230 (UTRN Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-NtC60αESNtLC60β
Plasmid#160878PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli.DepositorInsertrbcL, cpn60α, cpn60β, groES
UseTagsExpressionBacterialMutationPromoterrbcL: T7, chaperones: T7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NtLNtC60αESC60β
Plasmid#160875PurposeExpression of N. tabacum Rubisco large subunit (rbcL), Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coliDepositorInsertrbcL, cpn60α, cpn60β, groES
UseTagsExpressionBacterialMutationPromoterrbcL: pBAD, chaperones: T7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-Sox7
Plasmid#50780Purposeretroviral expression of mouse Sox7DepositorInsertSox7 (Sox7 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTS1051-Tier1-PhCMV-4xCox8
Plasmid#169490PurposeTier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)DepositorInsertPCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only