We narrowed to 1,383 results for: ccdB

1. pDGE1108

   Plasmid

   #202772

   Purpose

   CRISPR-KO in Arabidopsis thaliana

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

   Inserts

   FAST marker
   pRPS5a:zCas9_ttriple
   ccdB cassette (BsaI-excisable)
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKEB31

   Plasmid

   #31224

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   ccdB and Cm resistance gene
   

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOCC154

   Plasmid

   #118902

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p413TEF1-ATR1(pYL573)

   Plasmid

   #178288

   Purpose

   Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   ATR1
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOCC153

   Plasmid

   #118901

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDrop

   Plasmid

   #184872

   Purpose

   Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAL3

   Plasmid

   #114673

   Purpose

   T7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.

   Depositor

   William Studier

   Article

   Studier et al Curr Protoc Mol Biol. 2018 Oct;124(1):e6

   Type

   Empty backbone

   Tags

   optional Ser-Gly-6His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdestMB14

   Plasmid

   #26415

   Depositor

   Marc Vidal

   Article

   Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

   Insert

   attR4-ccdB-attR2
   

   Expression

   Bacterial and Worm

   Available Since

   April 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdestDD03

   Plasmid

   #26414

   Depositor

   Marc Vidal

   Article

   Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

   Insert

   attR4-ccdB-attR2 cassette
   

   Expression

   Bacterial and Worm

   Available Since

   April 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. BPK2014-AmCas12b

    Plasmid

    #121951

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AmCas12b
    

    Tags

    10xHis tag

    Expression

    Bacterial

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG415Gal_Gcn4 WW+GFP

    Plasmid

    #233003

    Purpose

    Galactose iduced expression of Gcn4 WW+GFP in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WW+
    

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    E109N, S117D, T121W, N126W, T132W

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG415Gal_Gcn4 Aro2+GFP

    Plasmid

    #233001

    Purpose

    Galactose iduced expression of Gcn4 Aro2+GFP in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 Aro2+
    

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    N116F, S117E, E119N, P129Q, T132Y

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG415Gal_Gcn4 solvvol

    Plasmid

    #232976

    Purpose

    Galactose iduced expression of Gcn4 solvvol in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 solvvol
    

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG415Gal_Gcn4 S/ArotoL

    Plasmid

    #232979

    Purpose

    Galactose iduced expression of Gcn4 S/ArotoL in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 S/ArotoL
    

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S101L, S104L, F108L, Y110L, S117L, S136L, S144L

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG415Gal_Gcn4 ILVtoED WHA

    Plasmid

    #232995

    Purpose

    Galactose iduced expression of Gcn4 ILVtoED W+HAin yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED W+
    

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    N126W, I128E, V130E, V135E, L137D, I142D

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG415Gal_Gcn4 9acidAHA

    Plasmid

    #232997

    Purpose

    Galactose iduced expression of Gcn4 9acidAHA in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 9acidA
    

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG415Gal_Gcn4 9acidAGFP

    Plasmid

    #233009

    Purpose

    Galactose iduced expression of Gcn4 9acidAGFP in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 9acidA
    

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG415Gal_Gcn4 Aro3+GFP

    Plasmid

    #233002

    Purpose

    Galactose iduced expression of Gcn4 Aro3+GFP in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 Aro3+
    

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    D103Y, N112T, L113Y, S117F, K140R

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG415Gal_Gcn4 ILVtoED WGFP

    Plasmid

    #233007

    Purpose

    Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED W+
    

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    N126W, I128E, V130E, V135E, L137D, I142D

    Promoter

    Gal1

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDGE1176

    Plasmid

    #202774

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge