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Plasmid#36875DepositorInserttTA2
UseTagsExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLMB714
Plasmid#83786PurposeC4-dicarboxylates biosensor plasmidDepositorInsertRegion upstream RL3424 (dctA)
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
P-d22bp
Plasmid#235657PurposeB. thetaiotaomicron fusA2 expression reporter lacking 22bp Cur binding siteDepositorInsertBacteroides thetaiotaomicron fusA2 promoter lacking the 22bp Cur binding site
UseTagsExpressionMutationThe B. thetaiotaomicron fusA2 promoter lacking &q…PromoterAvailable sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG059
Plasmid#231325PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RSF1010-Tc-par-ELT4; GG assembly from pOGG004, pOGG042, pNDGG005, pOGG012, and pOGG014, TcRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_CAG-rtTA-T2A-mTagBFP2
Plasmid#229797PurposeBxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2DepositorInsertrtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB
Plasmid#225488PurposeTet-on lentiviral vector for controlled expression of GDNFDepositorInsertTetracycline-controlled transactivator fused with Krüppel-associated box (KRAB) domain following an internal ribosome entry site
UseLentiviralTagsExpressionMammalianMutationPromoterMouse phosphoglycerate kinase 1Available sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRV∆L-5tTA
Plasmid#182966PurposeThe 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA)DepositorInserttTA
UseRabiesTagsExpressionMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
RVD-pG8
Plasmid#173343PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorInsertNN monomerRVD at Position8
UseUnspecifiedTagsExpressionMutationnoPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAP
Plasmid#169588PurposeTier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).DepositorInserttetracycline-controlled SEAP expression
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVmin-2Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH255-Tier1-OTetO7-PCMVmin1-SEAP
Plasmid#169587PurposeTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-1-SEAP-pA).DepositorInserttetracycline-controlled SEAP expression
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVmin-1Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
JGI_018357
Plasmid#158856PurposeRare earth element alcohol dehydrogenasesDepositorInsert31/32
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mRuby2
Plasmid#111250PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)DepositorInsertmRuby2
UseTagsExpressionBacterialMutationmRuby2 is codon optimized for expression in P. fl…PromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pS548∙CsR
Plasmid#197859PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Tc resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-tRNA2.0
Plasmid#158393PurposeGolden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAXEF-Rap1-WT
Plasmid#32697DepositorInsertRAP1 (RAP1A Human)
UseTagsExpressionMammalianMutationPromoterEF promoterAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV_hEF1a_rtTA3
Plasmid#61472Purpose3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)DepositorInsertrtTA3
UseLentiviralTagsExpressionMutationPromoterhEF1aAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131B2.0
Plasmid#99885PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132B2.0
Plasmid#99888PurposeGolden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + filler DNA reporter plasmid
Plasmid#156454PurposeA mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.DepositorInsertsNeoR/KanR
TcR
UseTagsExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7 and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133B2.0
Plasmid#99892PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only