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EK0718 SFFV-mCherry-SGc(s70587.os20)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247273

Purpose

modulADAR sensor against sythetic target transcript (36nt GABRA3 stem-loop)

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

mCherry-s70587.os20-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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EK0717 SFFV-mCherry-SGc(s70587.os19)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247272

Purpose

modulADAR sensor against sythetic target transcript (41nt GluR-B stemloop)

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

mCherry-s70587.os19-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK241 SFFV-mCherry-SGc(os13.NOT-SMN2.45,55,45)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247282

Purpose

modulADAR sensor for NOT SMN exon 7

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv [Preprint]. 2025 Sep 18:2025.09.

Insert

mCherry-os13.NOT-SMN2.45,55,45-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-TurboID

Plasmid

#245241

Purpose

Proximity-labeling proteomics negative control

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

TurboID

Expression

Mammalian

Mutation

wild-type

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-Spol-Ovo-Nterm

Plasmid

#242942

Purpose

Encodes an N-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

Spol-Ovo (Nterm fragment)

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-Spol-Ovo-C-term

Plasmid

#242943

Purpose

Encodes a C-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

Spol-Ovo (Cterm fragment)

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TPOR-LgBiT Vector

Plasmid

#238781

Purpose

Express TPOR-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

TPOR (MPL Human)

Tags

SmBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBD_PYR1_156

Plasmid

#241285

Purpose

Backbone for PYR1 sensor library assembly from oligopools; domesticated for golden gate cloning

Depositor

Sean Cutler

Article

Tian et al bioRxiv [Preprint]. 2025 May 15:2025.05.

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1057

Plasmid

#209547

Purpose

BTK assembled plasmid for integrating a KanR+GFP cassette into the S. alvi genome at a neutral site

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region

Use

Synthetic Biology

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1058

Plasmid

#209548

Purpose

BTK Type 1 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Upstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)

Use

Synthetic Biology

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-sncb-KALTA4

Plasmid

#239986

Purpose

Expresses GAL4 under control of the sncb promoter

Depositor

Summer Thyme

Article

Moyer et al bioRxiv [Preprint]. 2025 Mar 5:2025.02.2

Inserts

KALTA4
EGFP

Use

Zebrafish tol2/phic31 compatible

Promoter

myl7 and sncb

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC(h)-DEST-mKOk

Plasmid

#224830

Purpose

Empty Gateway DEST vector for expressing C-terminal tagged proteins with mKusabiraOrangekand hygromycin selection in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Type

Empty backbone

Tags

mKusabiraOrangek

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-stmn1b-KALTA4

Plasmid

#239987

Purpose

Expresses GAL4 under control of the stmn1b promoter

Depositor

Summer Thyme

Article

Moyer et al bioRxiv [Preprint]. 2025 Mar 5:2025.02.2

Inserts

KALTA4
EGFP

Use

Zebrafish tol2/phic31 compatible

Promoter

myl7 and stmn1b

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.6

Plasmid

#235471

Purpose

Message phagemid carrying sgRNA6 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA6

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.1

Plasmid

#235466

Purpose

Message phagemid carrying sgRNA1 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA1

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.2

Plasmid

#235467

Purpose

Message phagemid carrying sgRNA2 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA2

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.3

Plasmid

#235468

Purpose

Message phagemid carrying sgRNA3 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.4

Plasmid

#235469

Purpose

Message phagemid carrying sgRNA4 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA4

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.5

Plasmid

#235470

Purpose

Message phagemid carrying sgRNA5 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA5

Use

Synthetic Biology

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WT 30mer

Plasmid

#231888

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WT 30mer

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 30mer

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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