We narrowed to 13,244 results for: BASE

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pVD1 tRNA-gRNA position E2 (GB2239)

Plasmid

#160561

Purpose

tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tRNA-gRNA position E2 (Multiplexing Edit)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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Epac-S-H189

Plasmid

#170350

Purpose

mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

Insert

mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) (RAPGEF3 Human)

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE-BI GFP10-RHOA/RBD-GFP11

Plasmid

#182232

Purpose

Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Inserts

ras homolog family member A (RHOA Human)
rhotekin RHO binding domain (RBD)

Use

Lentiviral

Tags

GFP10 (split-GFP tripartite) and GFP11 (M4)

Promoter

TRE bidirectional

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDL52 (clone45)

Plasmid

#188578

Purpose

High-yield production of coenzyme F420 in E. coli

Depositor

Gerald Lackner

Article

Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

Inserts

ribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE

Expression

Bacterial

Mutation

Codon optimization, synthetic ribosome binding si…

Promoter

T7 variants

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLVNP2.0-SpCas9

Plasmid

#206880

Purpose

Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

SpCas9 (cas9 )

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

Plasmid

#160628

Purpose

Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos, 35S, 35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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FHL-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129628

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

FHL-cpmTq2-Calcium-lifetime-sensor

Tags

6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial and Mammalian

Promoter

CMV,&Rhamnose

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-P2A-GFP-PGK-Puro

Plasmid

#110866

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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ACTB-(A)4x

Plasmid

#112058

Purpose

ACTB mRNA tagged with 4 copies of Riboglow (variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

ACTB (ACTB Human)

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

4 copies of Riboglow tag after stop codon

Promoter

CMV

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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AAV_Efs_hSpCas9_NLS_FLAG-SV40

Plasmid

#97307

Purpose

AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

AAV and Mouse Targeting

Tags

Flag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AT-U1

Plasmid

#112059

Purpose

U1 snRNA tagged with 1 copy of Riboglow (truncated variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

U1 (RNU1-1 Human)

Expression

Mammalian

Mutation

1 copy of Riboglow tag (truncated variant A) near…

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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AAV_Cdx2 HMEJ donor

Plasmid

#97319

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Cdx2 HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6_YAP1-V5

Plasmid

#173002

Purpose

constitutive expression of V5-tagged YAP1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

YAP1-V5 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-FOG1[N+C]

Plasmid

#100085

Purpose

dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

FOG1(aa 1-45) (ZFPM1 Human)

Use

CRISPR

Tags

3XFLag-NLS-FOG1-dCas9-FOG1-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CamkII-bPac(WT)-mCherry-minWPRE.sbd

Plasmid

#118278

Purpose

Expresses bPAC(WT)-mCherry under a minimal CamKII Promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Insert

bPAC(WT)

Use

AAV

Tags

mCherry and myc

Promoter

CamKII

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HR donor

Plasmid

#97317

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-XRCC1-EGFP-Hygro

Plasmid

#176062

Purpose

EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

X-ray repair cross complementing 1 (XRCC1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

Plasmid

#97313

Purpose

Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

Lentiviral and Mouse Targeting

Tags

Flag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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PEA1-Nuclease-Puro

Plasmid

#171992

Purpose

Delivers all prime editing nuclease components in a single, puromycin selectable plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Mutation

GC to CA point mutation in SpCas9 to restore nucl…

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEZYeGFP-SARS-CoV-2E

Plasmid

#224580

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E (E SARS-CoV-2)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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