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pGS_107

Plasmid

#160650

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Publication

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAG32-3HA-3FLAG

Plasmid

#154081

Purpose

For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

Depositor

Cornelia Kilchert

Publication

Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

Insert

3xHA-3xFLAG-hygR

Use

Pcr-based tagging in yeast

Tags

3xHA-3xFLAG

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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ClhN-HIS5

Plasmid

#207014

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-MET15

Plasmid

#207013

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-URA3

Plasmid

#207012

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pKdH_P-GAP-ScACS1*

Plasmid

#126736

Purpose

yeast plasmid overexpressing ACS1 from Saccharomyces cerevisiae

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

acs1*

Tags

6xHis,Myc

Expression

Yeast

Mutation

T3542C and A30S, P113S, and S629N (please see dep…

Promoter

GAP

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pKdH_P-GAP-ScADH2

Plasmid

#126734

Purpose

yeast plasmid overexpressing ADH2 from Saccharomyces cerevisiae

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh2

Tags

6xHis

Expression

Yeast

Promoter

GAP

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*

Plasmid

#126738

Purpose

yeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiae

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh2,acs1*

Tags

6xHis,Myc

Expression

Yeast

Mutation

T3542C

Promoter

GAP

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pKdH_P-GAP-ScADH2+P-GAP*-ScALD6

Plasmid

#126737

Purpose

yeast plasmid overexpressing ADH2 and ALD6 from Saccharomyces cerevisiae

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh2,ald6

Tags

6xHis

Expression

Yeast

Promoter

GAP

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-ALD1

Plasmid

#126711

Purpose

yeast plasmid complementing ALD1 from Komagataella phaffii

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

ald1

Tags

6xHis,Myc

Expression

Yeast

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-ADH3

Plasmid

#126710

Purpose

yeast plasmid complementing ADH3 from Komagataella phaffii

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh3

Tags

6xHis,Myc

Expression

Yeast

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pK_P-GAP-LM_lacO-cP-AOX1-sAR

Plasmid

#126745

Purpose

yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

slovA,cpr

Tags

6xHis

Expression

Yeast

Promoter

GAP

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pK_P-ICL1-LM_lacO-cP-AOX1-sAR

Plasmid

#126744

Purpose

yeast plasmid overexpressing sLovA,CPR and LacI-Mit1AD

Depositor

Menghao Cai

Publication

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

slovA,cpr

Tags

6xHis

Expression

Yeast

Promoter

ICL1

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGS_107

Plasmid

#160650

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Publication

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG32-3HA-3FLAG

Plasmid

#154081

Purpose

For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

Depositor

Cornelia Kilchert

Publication

Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

Insert

3xHA-3xFLAG-hygR

Use

Pcr-based tagging in yeast

Tags

3xHA-3xFLAG

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Sc Pol3-FLAG(Ec)-pRSF405/GAL

Plasmid

#239198

Purpose

Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)

Depositor

Michael O'Donnell

Insert

Pol3 (POL3 Budding Yeast)

Tags

3X FLAG

Expression

Yeast

Mutation

E. coli codon bias (synthetic gene), but same ami…

Available since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAL005

Plasmid

#233865

Purpose

MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2

Depositor

Jeff Hasty

Publication

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2

Expression

Yeast

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMK296 (DHC1-mAID Neo)

Plasmid

#140541

Purpose

DHC1 tagging with mAID

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK297 (DHC1-mAID Hygro)

Plasmid

#140542

Purpose

DHC1 tagging with mAID

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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BAS287 C terminal E2C hygBR

Plasmid

#74082

Purpose

S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.

Depositor

Bassem Al-Sady

Publication

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

E2Crimson

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only