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pJZC545

Plasmid

#62313

Purpose

sgRNA with 1x MS2 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x MS2 binding module

Expression

Yeast

Promoter

SNR52

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMT Draper-Ala11-GFP

Plasmid

#126654

Purpose

Inducible expression of Draper-Ala11-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmi Tyr residues mutated to Ala

Expression

Insect

Mutation

Cytoplasmic Tyr residues to Ala

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P31

Plasmid

#120042

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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CytERM-dTomato

Plasmid

#98834

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

dTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-12

Plasmid

#119991

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFH2.21_mCherry-MS2x6

Plasmid

#205536

Purpose

Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a_speG_Cterm_His

Plasmid

#220483

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Zeo-ultraID

Plasmid

#207704

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-ultraID cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a_PA4114_Cterm_His

Plasmid

#220484

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA4-lenti

Plasmid

#115663

Purpose

Expresses AcrVA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPy-CAG-GFP-IRES-Pac

Plasmid

#48759

Purpose

CAG promoter driving GFP-IRES-Puromycin Acetyl Transferase

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

EGFP

Use

Ppy

Expression

Mammalian

Promoter

CAG

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-AlphaPix

Plasmid

#129609

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

Alpha-PIX

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-GST-eIF4G1-His6

Plasmid

#37231

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF4631 (TIF4631 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

tac

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-Ssh1

Plasmid

#163602

Purpose

Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-YOD1

Plasmid

#49784

Purpose

Express YOD1 with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Tags

Flag-tag

Mutation

WT

Available Since

Dec. 16, 2013

Availability

Academic Institutions and Nonprofits only
pRVdG-mCherry-N-P-M-EGFP-SynPhRFP-L

Plasmid

#52481

Purpose

Expresses mCherry, EGFP and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mCherry
EGFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pPRIME-TREX-GFP-recipient

Plasmid

#11661

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with Tet-responsive promoter (TREX) controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST17-Nanog

Plasmid

#13988

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

Nanog (Nanog Mouse)

Tags

His

Expression

Bacterial

Mutation

None

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pENTR SD Age HsSAS-6

Plasmid

#46381

Purpose

For expression of C-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Entry vector

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miniTurbo-Zeo

Plasmid

#207736

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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