We narrowed to 6,580 results for: Pet2;

1. pMSP1D12W/2Y

   Plasmid

   #124275

   Purpose

   Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence

   Depositor

   Alexey S. Ladokhin

   Insert

   Membrane scaffold protein
   

   Tags

   7xHis-tag

   Expression

   Bacterial

   Mutation

   Two tryptophans changed to two tyrosines

   Promoter

   T7

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDC32

   Plasmid

   #171204

   Purpose

   bacterial expression for HIS purification of aa1-698 of MEG-3

   Depositor

   Geraldine Seydoux

   Article

   Schmidt et al bioRxiv 2020.10.15.340570

   Insert

   meg-3 (meg-3 Nematode)
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   aa1-698

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAP20

   Plasmid

   #171206

   Purpose

   bacterial expression for HIS purification of the MEG-3 C-terminus

   Depositor

   Geraldine Seydoux

   Article

   Schmidt et al bioRxiv 2020.10.15.340570

   Insert

   meg-3 (meg-3 Nematode)
   

   Tags

   8xHIS

   Expression

   Bacterial

   Mutation

   aa545-862

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmSod1-Q42L;Q123A-N’His

   Plasmid

   #117710

   Purpose

   mutation on stability/solubility of SOD1

   Depositor

   Elizabeth Fisher

   Insert

   Sod1 (Sod1 Mouse)
   

   Tags

   His tag with Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Q42L; Q123A

   Promoter

   T7

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK611

   Plasmid

   #72532

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Expression

   Bacterial

   Promoter

   T7 and n/a

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK626

   Plasmid

   #72461

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to cysteine, tyrosine-…

   Promoter

   T7

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK691

   Plasmid

   #72381

   Purpose

   Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)

   Depositor

   T. J. Kappock

   Insert

   stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK292

   Plasmid

   #73371

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changed histidine-59 to asparagine and histidine-…

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK293

   Plasmid

   #73372

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changed histidine-59 to phenylalanine and histidi…

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK294

    Plasmid

    #73373

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changed histidine-59 to aspartate and histidine-8…

    Promoter

    T7

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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